Metabolic engineering for improved fermentation of pentoses by yeasts
@article{Jeffries2004MetabolicEF, title={Metabolic engineering for improved fermentation of pentoses by yeasts}, author={Thomas W. Jeffries and Y.-S Jin}, journal={Applied Microbiology and Biotechnology}, year={2004}, volume={63}, pages={495-509} }
Abstract The fermentation of xylose is essential for the bioconversion of lignocellulose to fuels and chemicals, but wild-type strains of Saccharomyces cerevisiae do not metabolize xylose, so researchers have engineered xylose metabolism in this yeast. Glucose transporters mediate xylose uptake, but no transporter specific for xylose has yet been identified. Over-expressing genes for aldose (xylose) reductase, xylitol dehydrogenase and moderate levels of xylulokinase enable xylose assimilation…
481 Citations
Improvement of xylose fermentation in respiratory-deficient xylose-fermenting Saccharomyces cerevisiae.
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Transcription analysis of recombinant Saccharomyces cerevisiae reveals novel responses to xylose
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It is suggested that cells metabolizing xylose are not in a completely repressed or in a derepressed state either, indicating thatxylose was recognized neither as a fermentable nor as a respirative carbon source.
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Additional evolutionary engineering was used to improve the fermentation kinetics of mixed-substrate utilisation, resulting in efficient D-xylose utilisation in synthetic media, and strain robustness, especially with respect to tolerance to inhibitors present in hydrolysates, can still be further improved.
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- Biology, EngineeringIndian Journal of Microbiology
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The genes coding xylose reductase and xylitol dehydrogenase from Pichia stipitis were successfully engineered into Saccharomyces cerevisae and engineering of XYL1 and XYL2 into yeasts significantly increased the microbial biomass and ethanol yield.
Ethanol production from xylose in engineered Saccharomyces cerevisiae strains: current state and perspectives
- Biology, EngineeringApplied Microbiology and Biotechnology
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In this review, recent progress with regard to studies using several promising approaches such as host strain selection and adaptation to obtain further improved xylose-utilizing S. cerevisiae strains are addressed.
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- Engineering, BiologyProceedings of the National Academy of Sciences
- 2010
Improved yields and productivities from cofermentation experiments performed with simulated cellulosic hydrolyzates are observed, suggesting this is a promising coferment strategy for cellulosIC biofuel production.
Expression of Vitreoscilla hemoglobin improves the metabolism of xylose in recombinant yeast Saccharomyces cerevisiae under low oxygen conditions
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Effects of xylitol dehydrogenase (XYL2) on xylose fermentation by engineered Candida glycerinogenes
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It was found that additional overexpression of XYL2 under the control of strong promoters in a xylose‐fermenting strain not only reduced xylitol accumulation but also increased glycerol yields.
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