Metabolic engineering for improved fermentation of pentoses by yeasts

@article{Jeffries2004MetabolicEF,
  title={Metabolic engineering for improved fermentation of pentoses by yeasts},
  author={Thomas W. Jeffries and Y.-S Jin},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={63},
  pages={495-509}
}
  • T. Jeffries, Y.-S Jin
  • Published 1 February 2004
  • Biology, Medicine
  • Applied Microbiology and Biotechnology
Abstract The fermentation of xylose is essential for the bioconversion of lignocellulose to fuels and chemicals, but wild-type strains of Saccharomyces cerevisiae do not metabolize xylose, so researchers have engineered xylose metabolism in this yeast. Glucose transporters mediate xylose uptake, but no transporter specific for xylose has yet been identified. Over-expressing genes for aldose (xylose) reductase, xylitol dehydrogenase and moderate levels of xylulokinase enable xylose assimilation… 
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Highly Influential Citations
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175
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479 Citations

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Improvement of xylose fermentation in respiratory-deficient xylose-fermenting Saccharomyces cerevisiae.
TLDR
Although overproduction of non-oxidative pentose phosphate pathway significantly increased the aerobic-specific growth rate on xylose and slightly improved conversion of xylOSE to ethanol under oxygen-limited conditions, the elimination of respiration by deleting cytochrome C oxidase subunit IV gene impeded aerobic growth onxylose.
Transcription analysis of recombinant Saccharomyces cerevisiae reveals novel responses to xylose
TLDR
It is suggested that cells metabolizing xylose are not in a completely repressed or in a derepressed state either, indicating thatxylose was recognized neither as a fermentable nor as a respirative carbon source.
Development of efficient xylose fermentation in Saccharomyces cerevisiae: xylose isomerase as a key component.
TLDR
Additional evolutionary engineering was used to improve the fermentation kinetics of mixed-substrate utilisation, resulting in efficient D-xylose utilisation in synthetic media, and strain robustness, especially with respect to tolerance to inhibitors present in hydrolysates, can still be further improved.
Comparative metabolic network analysis of two xylose fermenting recombinant Saccharomyces cerevisiae strains.
TLDR
The metabolic network analysis with 13C labelled glucose showed that there was a shift in the specific xylose reductase activity towards use of NADH as co-factor rather than NADPH, which can therefore partly explain the 25% increase in the ethanol yield observed for CPB.CR4.
Strain engineering of Saccharomyces cerevisiae for enhanced xylose metabolism.
TLDR
Vibrant and persistent researches in this field for the last two decades not only led to the development of engineered S. cerevisiae strains ready for industrial fermentation of cellulosic hydrolysates, but also deepened the understanding of operational principles underlying yeast metabolism.
Metabolic engineering of Saccharomyces cerevisiae for increased bioconversion of lignocellulose to ethanol
TLDR
The genes coding xylose reductase and xylitol dehydrogenase from Pichia stipitis were successfully engineered into Saccharomyces cerevisae and engineering of XYL1 and XYL2 into yeasts significantly increased the microbial biomass and ethanol yield.
Ethanol production from xylose in engineered Saccharomyces cerevisiae strains: current state and perspectives
TLDR
In this review, recent progress with regard to studies using several promising approaches such as host strain selection and adaptation to obtain further improved xylose-utilizing S. cerevisiae strains are addressed.
Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation
TLDR
Improved yields and productivities from cofermentation experiments performed with simulated cellulosic hydrolyzates are observed, suggesting this is a promising coferment strategy for cellulosIC biofuel production.
Expression of Vitreoscilla hemoglobin improves the metabolism of xylose in recombinant yeast Saccharomyces cerevisiae under low oxygen conditions
TLDR
Results indicate that under low hypoxic fermentation conditions VHb improves the ethanol yield from the xylose metabolized, and less carbon was lost in xylitol, which is the major unwanted side product of thexylose-to-ethanol fermentation process.
Effects of xylitol dehydrogenase (XYL2) on xylose fermentation by engineered Candida glycerinogenes
TLDR
It was found that additional overexpression of XYL2 under the control of strong promoters in a xylose‐fermenting strain not only reduced xylitol accumulation but also increased glycerol yields.
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  • Biology, Medicine
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TLDR
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