Metabolic effects on recombinant interferon-gamma glycosylation in continuous culture of Chinese hamster ovary cells

@article{Nyberg1999MetabolicEO,
  title={Metabolic effects on recombinant interferon-gamma glycosylation in continuous culture of Chinese hamster ovary cells},
  author={Gregg B. Nyberg and R. Robert Balcarcel and Brian D. Follstad and Gregory Stephanopoulos and Daniel I. C. Wang},
  journal={Biotechnology and bioengineering},
  year={1999},
  volume={62 3},
  pages={336-47}
}
Asparagine linked (N-linked) glycosylation is an important modification of recombinant proteins, because the attached oligosaccharide chains can significantly alter protein properties. Potential glycosylation sites are not always occupied with oligosaccharide, and site occupancy can change with the culture environment. To investigate the relationship between metabolism and glycosylation site occupancy, we studied the glycosylation of recombinant human interferon-gamma (IFN-gamma) produced in… CONTINUE READING

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