Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives.

@article{Noda2016MetabolicDO,
  title={Metabolic design of a platform Escherichia coli strain producing various chorismate derivatives.},
  author={Shuhei Noda and Tomokazu Shirai and Sachiko Oyama and Akihiko Kondo},
  journal={Metabolic engineering},
  year={2016},
  volume={33},
  pages={119-129}
}
A synthetic metabolic pathway suitable for the production of chorismate derivatives was designed in Escherichia coli. An L-phenylalanine-overproducing E. coli strain was engineered to enhance the availability of phosphoenolpyruvate (PEP), which is a key precursor in the biosynthesis of aromatic compounds in microbes. Two major reactions converting PEP to pyruvate were inactivated. Using this modified E.coli as a base strain, we tested our system by carrying out the production of salicylate, a… CONTINUE READING