Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content

@article{Weselake2008MetabolicCA,
  title={Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content},
  author={Randall J Weselake and Saleh Shah and Mingguo Tang and Patti A. Quant and Crystal L. Snyder and Tara L. Furukawa-Stoffer and Weiming Zhu and David McD Taylor and Jitao Zou and Arvind Kumar and Linda M. Hall and Andr{\'e} Laroche and G. F. W. Rakow and Phillip Raney and Maurice M. Moloney and John L Harwood},
  journal={Journal of Experimental Botany},
  year={2008},
  volume={59},
  pages={3543 - 3549}
}
Top-down control analysis (TDCA) is a useful tool for quantifying constraints on metabolic pathways that might be overcome by biotechnological approaches. Previous studies on lipid accumulation in oilseed rape have suggested that diacylglycerol acyltransferase (DGAT), which catalyses the final step in seed oil biosynthesis, might be an effective target for enhancing seed oil content. Here, increased seed oil content, increased DGAT activity, and reduced substrate:product ratio are demonstrated… CONTINUE READING
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