Metabolic biotinylation of lentiviral pseudotypes for scalable paramagnetic microparticle-dependent manipulation.

@article{Nesbeth2006MetabolicBO,
  title={Metabolic biotinylation of lentiviral pseudotypes for scalable paramagnetic microparticle-dependent manipulation.},
  author={Darren N Nesbeth and Sharon L. Williams and Lucas Chan and Tony Brain and Nigel K H Slater and Farzin Farzaneh and D. Christopher Darling},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2006},
  volume={13 4},
  pages={814-22}
}
Nonviral, host-derived proteins on lentiviral vector surfaces can have a profound effect on the vector's biology as they can both promote infection and provide resistance to complement inactivation. We have exploited this to engineer a specific posttranslational modification of a "nonenvelope," virally associated protein. The bacterial biotin ligase (BirA) and a modified human DeltaLNGFR have been introduced into HEK293T cells and their protein products directed to the lumen of the endoplasmic… CONTINUE READING
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