Metabolic and signaling properties of an Itpk gene family in Glycine max.

@article{Stiles2008MetabolicAS,
  title={Metabolic and signaling properties of an Itpk gene family in Glycine max.},
  author={Amanda R. Stiles and Xun Qian and Stephen B Shears and Elizabeth A. Grabau},
  journal={FEBS letters},
  year={2008},
  volume={582 13},
  pages={1853-8}
}
We have cloned and characterized four Itpk genes from soybean. All four recombinant Itpk proteins showed canonical Ins(1,3,4)P3 5/6-kinase activity, but a kinetic analysis raised questions about its biological significance. Instead, we provide evidence that one alternative biological role for soybean Itpks is to interconvert the Cl(-) channel inhibitor, Ins(3,4,5,6)P4, and its metabolic precursor, Ins(1,3,4,5,6)P5, within a substrate cycle. The soybean Itpks also phosphorylated Ins(3,4,6)P3 to… CONTINUE READING