Metabolic and regulatory rearrangements underlying efficient D-xylose utilization in engineered Pseudomonas putida S12.

@article{Meijnen2012MetabolicAR,
  title={Metabolic and regulatory rearrangements underlying efficient D-xylose utilization in engineered Pseudomonas putida S12.},
  author={Jean-Paul Meijnen and Johannes H. de Winde and Harald J. Ruijssenaars},
  journal={The Journal of biological chemistry},
  year={2012},
  volume={287 18},
  pages={14606-14}
}
Previously, an efficient D-xylose utilizing Pseudomonas putida S12 strain was obtained by introducing the D-xylose isomerase pathway from Escherichia coli, followed by evolutionary selection. In the present study, systemic changes associated with the evolved phenotype were identified by transcriptomics, enzyme activity analysis, and inverse engineering. A key element in improving the initially poor D-xylose utilization was the redistribution of 6-phospho-D-gluconate (6-PG) between the Entner… CONTINUE READING