Metabolic and Proteomic Profiling of Diapause in the Aphid Parasitoid

Abstract

Background: Diapause, a condition of developmental arrest and metabolic depression exhibited by a wide range of animals is accompanied by complex physiological and biochemical changes that generally enhance environmental stress tolerance and synchronize reproduction. Even though some aspects of diapause have been well characterized, very little is known about the full range of molecular and biochemical modifications underlying diapause in non-model organisms. Methodology/Principal Findings: In this study we focused on the parasitic wasp, Praon volucre that exhibits a pupal diapause in response to environmental signals. System-wide metabolic changes occurring during diapause were investigated using GC-MS metabolic fingerprinting. Moreover, proteomic changes were studied in diapausing versus nondiapausing phenotypes using a combination of two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry. We found a reduction of Krebs cycle intermediates which most likely resulted from the metabolic depression. Glycolysis was galvanized, probably to favor polyols biosynthesis. Diapausing parasitoids accumulated high levels of cryoprotective polyols, especially sorbitol. A large set of proteins were modulated during diapause and these were involved in various functions such as remodeling of cytoskeleton and cuticle, stress tolerance, protein turnover, lipid metabolism and various metabolic enzymes. Conclusions/Significance: The results presented here provide some first clues about the molecular and biochemical events that characterize the diapause syndrome in aphid parasitoids. These data are useful for probing potential commonality of parasitoids diapause with other taxa and they will help creating a general understanding of diapause underpinnings and a background for future interpretations. Citation: Colinet H, Renault D, Charoy-Guével B, Com E (2012) Metabolic and Proteomic Profiling of Diapause in the Aphid Parasitoid Praon volucre. PLoS ONE 7(2): e32606. doi:10.1371/journal.pone.0032606 Editor: Pedro Santos, University of Minho, Portugal Received December 1, 2011; Accepted January 28, 2012; Published February 28, 2012 Copyright: 2012 Colinet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Funding: This study was supported by Fonds de la Recherche Scientifique – FNRS in Belgium. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Competing Interests: The authors have declared that no competing interests exist. * E-mail: herve.colinet@uclouvain.be ¤ Current address: UMR CNRS 6553 Bât 14A, Université de Rennes 1, Rennes, France

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@inproceedings{volucre2012MetabolicAP, title={Metabolic and Proteomic Profiling of Diapause in the Aphid Parasitoid}, author={Praon volucre and Herv{\'e} Colinet and David Renault and Blandine Charoy-Gu{\'e}vel and Emmanuelle Com}, year={2012} }