Metabolic activity in primary cultures of fish hepatocytes.

  title={Metabolic activity in primary cultures of fish hepatocytes.},
  author={Helmut Segner and Jean Pierre Cravedi},
  journal={Alternatives to laboratory animals : ATLA},
  volume={29 3},
In aquatic toxicology, isolated liver cells from fish can be used as a tool to generate initial information on the hepatic metabolism of xenobiotics, and on the mechanisms of xenobiotic activation or deactivation. This isolation of teleost liver cells is achieved by enzymic dissociation, and monolayer cultures of fish hepatocytes in serum-free medium maintain good viability for 3-8 days. During in vitro culture, fish liver cells express stable levels of phase I and phase II enzymes, such as… CONTINUE READING

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