Metabolic activation and immunochemical localization of liver protein adducts of the nonsteroidal anti-inflammatory drug diclofenac.

@article{Hargus1994MetabolicAA,
  title={Metabolic activation and immunochemical localization of liver protein adducts of the nonsteroidal anti-inflammatory drug diclofenac.},
  author={Stefan Hargus and H R Amouzedeh and Neil R. Pumford and Timothy G. Myers and Sean C. McCoy and Lance R. Pohl},
  journal={Chemical research in toxicology},
  year={1994},
  volume={7 4},
  pages={575-82}
}
Diclofenac is a nonsteroidal anti-inflammatory agent that is reported to cause serious hepatic injury in some patients. To investigate the possibility that protein adducts derived from reactive intermediates of diclofenac might be responsible for the hepatotoxicity produced by this drug, we recently developed polyclonal antisera that recognized protein adducts of diclofenac. In the present study, we have characterized further the diclofenac adducts in rat liver. Immunoblotting studies showed… CONTINUE READING