Metabolic acidosis and the importance of balanced equations

@article{Lane2008MetabolicAA,
  title={Metabolic acidosis and the importance of balanced equations},
  author={Andrew N. Lane and Teresa W.-M. Fan and Richard M. Higashi},
  journal={Metabolomics},
  year={2008},
  volume={5},
  pages={163-165}
}
Balancing biochemical equations for both mass and charge in metabolic networks is critical but unfortunately ignored too often. Failure to do so, for example, results in a common misconception about the origin of protons during lactic fermentation. Lactate, rather than lactic acid, is produced by glycolysis, and its production is a mechanism for alleviating intracellular acidosis due to glycolysis. This error is at the core of some recent papers, and is often ambiguous in biochemistry textbooks… 
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31 Citations
Background Citations
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Methods Citations
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31 Citations

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    Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology
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TLDR
All single reactions and overall equations leading to the metabolic interaction between ureagenesis and citric acid cycle to be incorporated into a two‐three lecture series about the interaction of urea cycle with other metabolic pathways are presented.
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The classic view that most of the net proton efflux from C6 gliomas originates in glycolytic formation of lactate and H+ inside the tumor is supported, but add that some lactate is taken up into cells in the rim on MCT1, and some lactates diffuses away, leaving its associated protons available to re-enter cells for extrusion on NHE1.
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TLDR
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The in vivo results support a multipronged approach to tumor treatment based on minimizing intracellular pH by targeting several proton production and proton transport processes, among which the very efficient MCT4 proton/lactate co‐transport deserves particular attention.
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References

SHOWING 1-10 OF 16 REFERENCES
Acid production in glycolysis-impaired tumors provides new insights into tumor metabolism.
TLDR
Evidence is found supporting the hypothesis that tumor cells rely on glutaminolysis for energy production and that the pentose phosphate pathway is highly active within tumor cells and suggesting that the tricarboxylic acid cycle is saturable and that different metabolic pathways are activated to provide for energyproduction and biosynthesis.
The proton-linked monocarboxylate transporter (MCT) family: structure, function and regulation.
TLDR
There is still much work to be done to characterize the properties of the different MCT isoforms and their regulation, which may have wide-ranging implications for health and disease.
Why do cancers have high aerobic glycolysis?
TLDR
It is proposed that persistent metabolism of glucose to lactate even in aerobic conditions is an adaptation to intermittent hypoxia in pre-malignant lesions, which leads to microenvironmental acidosis requiring evolution to phenotypes resistant to acid-induced cell toxicity.
Intracellular pH regulation in leukocytes: mechanisms and functional significance.
TLDR
Several pHi regulatory mechanisms, including the sodium-proton exchanger, proton conductive pathways, and a vacuolar type H(+)-ATPase exist in leukocytes and play an important role in preventing deviations in the pHi away from the physiological range.
Molecular features, regulation, and function of monocarboxylate transporters: implications for drug delivery.
TLDR
The expression of MCT1 in the epithelium of the small intestine and colon and in the blood-brain barrier may provide routes for the intestinal and blood to brain transfer of carboxylated pharmaceutical agents and other exogenous monocarboxylates.
Lactic Acid--The Latest Performance-Enhancing Drug
TLDR
By altering the activity of chloride ion channels, lactic acid may actually boost the generation of action potentials and, hence, muscle activity even as the muscles begin to fatigue.
Intracellular pH measurements by 31P nuclear magnetic resonance. Influence of factors other than pH on 31P chemical shifts.
TLDR
The results clearly indicate that knowledge of intracellular ionic strength and free Mg2+ concentrations in the sample are required if the determination of intrACEllular pH by 31P NMR is to be considered accurate within +/- 0.05-01 pH unit.
An in vivo 1H and 31P NMR investigation of the effect of nitrate on hypoxic metabolism in maize roots.
Effect of cell spreading on cytoplasmic pH in normal and transformed fibroblasts.
TLDR
The results suggest that the requirement for spreading may in part be mediated by cytoplasmic pH, and Anchorage-independent growth due to oncogenes that localize to the plasma membrane is associated with loss of this control mechanism.
Regulation of cytoplasmic pH by Na+/H+ exchange
...
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