Metabolic Footprinting of a Clear Cell Renal Cell Carcinoma in Vitro Model for Human Kidney Cancer Detection.

@article{Knott2018MetabolicFO,
  title={Metabolic Footprinting of a Clear Cell Renal Cell Carcinoma in Vitro Model for Human Kidney Cancer Detection.},
  author={M. E. Knott and M. Manzi and Nicol{\'a}s Zabalegui and M. O. Salazar and L. Puricelli and M. Monge},
  journal={Journal of proteome research},
  year={2018},
  volume={17 11},
  pages={
          3877-3888
        }
}
A protocol for harvesting and extracting extracellular metabolites from an in vitro model of human renal cell lines was developed to profile the exometabolome by means of a discovery-based metabolomics approach using ultraperformance liquid chromatography coupled to quadrupole-time-of-flight mass spectrometry. Metabolic footprints provided by conditioned media (CM) samples ( n = 66) of two clear cell Renal Cell Carcinoma (ccRCC) cell lines with different genetic backgrounds and a nontumor renal… Expand