Metabolic Engineering in Nicotiana benthamiana Reveals Key Enzyme Functions in Arabidopsis Indole Glucosinolate Modification[W]

@article{Pfalz2011MetabolicEI,
  title={Metabolic Engineering in Nicotiana benthamiana Reveals Key Enzyme Functions in Arabidopsis Indole Glucosinolate Modification[W]},
  author={Marina Pfalz and Michael Dalgaard Mikkelsen and Paweł Bednarek and Carl Erik Olsen and Barbara Ann Halkier and Juergen Kroymann},
  journal={Plant Cell},
  year={2011},
  volume={23},
  pages={716 - 729}
}
Chemical modifications such as hydroxylations or methylations can alter the ecological significance of plant secondary metabolites. This work shows that members from two gene families, CYP81Fs and IGMTs, are responsible for such modifications of indole glucosinolates in Arabidopsis. Indole glucosinolates, derived from the amino acid Trp, are plant secondary metabolites that mediate numerous biological interactions between cruciferous plants and their natural enemies, such as herbivorous insects… Expand
Indole Glucosinolate Biosynthesis Limits Phenylpropanoid Accumulation in Arabidopsis thaliana
TLDR
The ref5-1 mutant and its suppressors reveal that an intermediate in indole glucosinolate biosynthesis impacts the early steps of phenylpropanoid metabolism, an interaction that requires the Mediator complex, and suggests that the function of the Mediation complex is required for the crosstalk. Expand
Conservation and clade-specific diversification of pathogen-inducible tryptophan and indole glucosinolate metabolism in Arabidopsis thaliana relatives.
TLDR
It is concluded that pathogen-inducible IG metabolism in the Brassicaceae is evolutionarily ancient, while other tryptophan-derived branch pathways represent relatively recent manifestations of a plant-pathogen arms race. Expand
Sulfur‐Containing Secondary Metabolites from Arabidopsis thaliana and other Brassicaceae with Function in Plant Immunity
  • P. Bednarek
  • Biology, Medicine
  • Chembiochem : a European journal of chemical biology
  • 2012
TLDR
It is likely that glucosinolates and Brassicaceae phytoalexins, traditionally considered as separate groups of compounds, have a common evolutionary origin and are interconnected on the biosynthetic level, and this suggests that the diversity of Br Jurassicaceae sulfur‐containing phytochemicals reflect phylogenetic clade‐specific branches of an ancient biosynthesis pathway. Expand
The Function of Glucosinolates and Related Metabolites in Plant Innate Immunity
TLDR
Experimental evidence indicate that some molecules released during pathogen-triggered glucosinolate metabolism may activate evolutionarily conserved immune responses, possibly through affecting glutathione redox state in the infected cell. Expand
Biosynthesis of cabbage phytoalexins from indole glucosinolate
TLDR
The identification of these biosynthetic enzymes and the heterologous reconstitution of the indole–sulfur phy toalexin pathway sheds light on an important pathway in an edible plant and opens the door to using metabolic engineering to systematically quantify the impact of cruciferous phytoalexins on plant disease resistance and human health. Expand
Methyl Transfer in Glucosinolate Biosynthesis Mediated by Indole Glucosinolate O-Methyltransferase 51
Indole glucosinolate O-methyltransferase 5 catalyzes the methyl transfer reaction in the biosynthesis of Trp-derived glucosinolates that are modified at position 1 of the indole ring. IndoleExpand
Regulatory and Functional Aspects of Indolic Metabolism in Plant Systemic Acquired Resistance.
TLDR
Investigation of the functional role and regulatory characteristics of indolic metabolism in Arabidopsis systemic acquired resistance (SAR) triggered by the bacterial pathogen Pseudomonas syringae suggests that systemically elevated indoles are dispensable for SAR and associated systemic increases of salicylic acid. Expand
Title The Glucosinolate Biosynthetic Gene AOP 2 Mediates Feedback Regulation of Jasmonic Acid Signaling in Arabidopsis Permalink
Survival in changing and challenging environments requires an organism to efficiently obtain and use its resources. Due to their sessile nature, it is particularly critical for plants to dynamicallyExpand
The role of CYP71A12 monooxygenase in pathogen-triggered tryptophan metabolism and Arabidopsis immunity.
TLDR
It is shown that both enzymes are key players in the resistance of Arabidopsis against selected filamentous pathogens after they invade and that low amounts of ICA can form during an immune response by CYP71B6/AAO1-dependent metabolism of indole acetonitrile, but not via IG hydrolysis. Expand
Natural variation of root exudates in Arabidopsis thaliana-linking metabolomic and genomic data
TLDR
The study elucidates the chemical composition of the rhizosphere and its natural variation in A. thaliana, which is important for the attraction and shaping of microbial communities, and identifies biosynthetic enzymes in metabolomics experiments. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 74 REFERENCES
The Gene Controlling the Indole Glucosinolate Modifier1 Quantitative Trait Locus Alters Indole Glucosinolate Structures and Aphid Resistance in Arabidopsis[W]
TLDR
CYP81F2 contributes to defense against the green peach aphid but not to resistance against herbivory by larvae from four lepidopteran species, and the IGM1 QTL is largely caused by differences in CYP81F1 expression. Expand
Benzoic acid glucosinolate esters and other glucosinolates from Arabidopsis thaliana.
TLDR
This work reinvestigated Columbia as well as additional ecotypes and mutant lines, and identified 12 further glucosinolates, including five novel compounds, which were the first non-chain elongated, methionine-derived glucos inolate from A. thaliana and the first compounds that appear to be derived from leucine. Expand
CYP83B1, a Cytochrome P450 at the Metabolic Branch Point in Auxin and Indole Glucosinolate Biosynthesis in Arabidopsis
TLDR
Indole-3-acetaldoxime is the metabolic branch point between the primary auxin indole- 3-acetic acid and indole glucosinolate biosynthesis in Arabidopsis, which leads to plants with a phenotype that suggests severe auxin overproduction. Expand
A Glucosinolate Metabolism Pathway in Living Plant Cells Mediates Broad-Spectrum Antifungal Defense
TLDR
It is proposed that reiterated enzymatic cycles, controlling the generation of toxic molecules and their detoxification, enable the recruitment of glucosinolates in defense responses. Expand
Omics-based identification of Arabidopsis Myb transcription factors regulating aliphatic glucosinolate biosynthesis
TLDR
Two R2R3-Myb transcription factors are found that positively control the biosynthesis of GSLs in Arabidopsis thaliana by an integrated omics approach and a working model for regulation of GSL production involving these genes, renamed Production of Methionine-Derived Glucosinolate (PMG) 1 and 2 are postulated. Expand
Positive selection driving diversification in plant secondary metabolism.
TLDR
The results show that gene duplication, neofunctionalization, and positive selection provide the mechanism for biochemical adaptation in plant defense and their fundamental importance for the evolution of plant metabolic diversity both within and among species. Expand
Genetic control of natural variation in Arabidopsis glucosinolate accumulation.
TLDR
There appears to be a modular genetic system regulating glucos inolate profiles in Arabidopsis that allows the rapid generation of new glucosinolate combinations in response to changing herbivory or other selective pressures. Expand
Arabidopsis glucosyltransferase UGT74B1 functions in glucosinolate biosynthesis and auxin homeostasis.
TLDR
Biochemical analyses demonstrate that recombinant UGT74B1 specifically glucosylates the thiohydroximate functional group, and promoter activity during plant development reveals expression patterns consistent with glucosinolate metabolism and induction by auxin treatment, providing complementary in vitro and in vivo evidence for a primary role of UGT 74B1 in glucosInolate biosynthesis. Expand
Camalexin is synthesized from indole-3-acetaldoxime, a key branching point between primary and secondary metabolism in Arabidopsis.
TLDR
The structural similarity between cruciferous indole phytoalexins suggests that these compounds are biogenetically related and synthesized from tryptophan via IAOx by CYP79B homologues, where IAOx has been shown to play a critical role in IAA homeostasis. Expand
A gene controlling variation in Arabidopsis glucosinolate composition is part of the methionine chain elongation pathway.
TLDR
The gene methylthioalkylmalate synthase1 (MAM1) is a member of a gene family sharing approximately 60% amino acid sequence similarity with 2-isopropylmalates synthase, an enzyme of leucine biosynthesis that condenses 2-oxo-3-methylbutanoate with acetyl-coenzyme A. Expand
...
1
2
3
4
5
...