Messenger RNA for the coat protein of tobacco mosaic virus

@article{Hunter1976MessengerRF,
  title={Messenger RNA for the coat protein of tobacco mosaic virus},
  author={Tony Hunter and Tim Hunt and John Knowland and David Zimmern},
  journal={Nature},
  year={1976},
  volume={260},
  pages={759-764}
}
TMV RNA is not an efficient template for translation of the viral coat protein, in spite of containing nucleo-tide sequences coding for the protein. Efficient translation requires the prior synthesis within infected cells of a smaller RNA carrying only a portion of the information encoded in the whole genome. 
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234 Citations
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Background Citations
28
Methods Citations
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Regulation of Translation of Viral mRNAs
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Viral genomes, and in particular viral RNA genomes, have served as model systems to investigate the steps involved in protein synthesis and have successfully helped to unravel the various strategies of translation known to occur on mRNA templates.
The Translation of Large Plant Viral RNAs
TLDR
The following overview of plant viral translation will center on the viruses with large, presumably multigenic RNAs (molecular weight over 106) and the large components of the multicomponent viruses like the bromoviridae.
The initiation site for transcription of the TMV 30‐kDa protein messenger RNA
Effects of the 5′-Leader Sequence of Tobacco Mosaic Virus RNA, or Derivatives Thereof, on Foreign mRNA and Native Viral Gene Expression
TLDR
One such pleiotropic sequence, the 5′-untranslated leader of tobacco mosaic virus (TMV) RNA, is the main subject of this article.
Virus reconstitution and the proof of the existence of genomic RNA.
This paper is a historical overview of the work done on the tobacco mosaic virus. The primary finding was that a virus is capable of reassembling itself from its component protein and RNA, and that…
Virus reconstitution and the proof of the existence of genomic RNA.
  • H. Fraenkel-conrat
  • Biology
    BioEssays : news and reviews in molecular, cellular and developmental biology
  • 1990
This paper is a historical overview of the work done on the tobacco mosaic virus. The primary finding was that a virus is capable of reassembling itself from its component protein and RNA, and that…
In vitro viral RNA synthesis by a subcellular fraction of TMV-inoculated tobacco protoplasts.
Efficient translation of the coat protein cistron of tobacco mosaic virus in a cell-free system from Escherichia coli.
TLDR
Translation of tobacco mosaic virus RNA in a cell-free system derived from Escherichia coli reveals several discrete polypeptides in the Mr range of 10,000-50,000 which comigrates with authentic TMV coat protein on sodium dodecyl sulphate/polyacrylamide gel electrophoresis.
Synthesis of TMV-specific RNAs and proteins at the early stage of infection in tobacco protoplasts: transient expression of the 30K protein and its mRNA.
Leaky UAG termination codon in tobacco mosaic virus RNA
TLDR
It is shown here that synthesis of these two proteins is initiated at the same site; the larger product is generated by partial readthrough of an amber (UAG) termination codon, and in the presence of yeast amber suppressor tRNA, the in vitro synthesis of the 160Kprotein is increased whereas the yield of the 110K protein is diminished.
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References

SHOWING 1-10 OF 46 REFERENCES
The coat protein gene of tobacco mosaic virus. I. Location of the gene by mixed infection.
Assembly of the particle of tobacco mosaic virus from RNA and disks of protein.
TLDR
The reconstitution of TMV does not proceed by the stepwise addition of single protein subunits, but by the addition of preformed disks to the growing rod, which is the basis of the selectivity for viral over other RNAs.
Origin of the small component of brome mosaic virus RNA.
Protein synthesis directed by the RNA from a plant virus in a normal animal cell.
RNA from tobacco mosaic virus can be translated inside oocytes of the frog Xenopus laevis. The main product is a polypeptide with a molecular weight of 140,000. There is no evidence for coat protein…
Nucleotide sequence of a viral RNA fragment that binds to eukaryotic ribosomes
The nucleotide sequence has been determined for the first 53 bases of brome mosaic virus RNA4, the monocistronic messenger for brome mosaic virus coat protein. The sequence includes the binding site…
Binding of histidine to tobacco mosaic virus RNA.
Translation of TMV-RNA in a cell-free wheat embryo system.
Tobacco mosaic virus RNA directs the synthesis of a coat protein peptide in a cell-free system from wheat.
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  • Biology, Medicine
    Journal of molecular biology
  • 1973
Protein synthesis in tobacco protoplasts infected with tobacco mosaic virus.
The 5' end group of tobacco mosaic virus RNA is m7G5' ppp5' Gp.
TLDR
TMV RNA, like many animal cellular and viral mRNAs recently examined, has a 5' terminus blocked by a methylated nucleotide inverted with respect to the rest of the chain, yielding products which imply the structure m7G5'ppp5'Gp.
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