Messenger RNA-based vaccines

@article{Pascolo2004MessengerRV,
  title={Messenger RNA-based vaccines},
  author={Steve Pascolo},
  journal={Expert Opinion on Biological Therapy},
  year={2004},
  volume={4},
  pages={1285 - 1294}
}
  • S. Pascolo
  • Published 1 August 2004
  • Biology
  • Expert Opinion on Biological Therapy
RNA is the only molecule known to recapitulate all biochemical functions of life: definition, control and transmission of genetic information, creation of defined three-dimensional structures, enzymatic activities and storage of energy. Because of its versatility and thanks to several recent scientific breakthroughs, RNA became the focus of intense research in molecular medicine at the beginning of the millennium. In particular, mRNA can be seen as a safe and efficient alternative to protein… 
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131 Citations
Highly Influential Citations
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Background Citations
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Methods Citations
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131 Citations

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References

SHOWING 1-10 OF 75 REFERENCES
In vitro-synthesized infectious RNA as an attenuated live vaccine in a flavivirus model
TLDR
This work proposes a novel live attenuated virus vaccination strategy consisting of the application of in vitro-synthesized infectious RNA instead of the live virus itself, which induced a protective immunity in laboratory mice.
Self-replicating Semliki Forest virus RNA as recombinant vaccine.
Emerging clinical applications of RNA
TLDR
The first generation of the resulting RNA therapeutics are now being evaluated in clinical trials, raising significant interest in this emerging area of medical research.
Immunostimulating capacities of stabilized RNA molecules
TLDR
It is shown that mRNA molecules that are protected from immediate degradation either through interaction with cationic proteins (trans protection) or through chemical modification of the phosphodiester backbone (phosphorothioate RNA; cis protection) act as sequence‐independent danger signals on mouse DC.
Technical and regulatory hurdles for DNA vaccines.
Cancer therapy using a self-replicating RNA vaccine
TLDR
The enhanced efficacy in vivo correlated with a caspase-dependent apoptotic death in transfected cells, providing a potential mechanism for enhanced immunogenicity.
Induction of virus‐specific cytotoxic T lymphocytes in vivo by liposome‐entrapped mRNA
TLDR
The induction of anti‐influenza cytotoxic T lymphocytes (CTL) in vivo by immunizing mice with liposomes containing messenger RNA (mRNA) encoding the influenza virus nucleoprotein (NP) is described and the relevance of these results in the context of vaccine development is discussed.
DNA vaccines: safety and efficacy issues
TLDR
This review will focus on whether DNA vaccines are likely to induce systemic or organ-specific autoimmune disease, have the potential to induce tolerance rather than immunity, and are as effective in individuals with depressed immune function as they are in healthy adults.
Microparticle-mediated RNA immunization against bovine viral diarrhea virus.
Genetic immunization is a simple method for eliciting an immune response
TLDR
It is reported that an immune response can be elicited by introducing the gene encoding a protein directly into the skin of mice by using a hand-held form of the biolistic system.
...
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