Mesenchymal Stem Cells from Bone Marrow Enhance Neovascularization and Stromal Cell Proliferation in Rat Ischemic Limb in the Early Phase after Implantation

  title={Mesenchymal Stem Cells from Bone Marrow Enhance Neovascularization and Stromal Cell Proliferation in Rat Ischemic Limb in the Early Phase after Implantation},
  author={Sayaka Usui and Yoshitaka Iso and Masahiro Sasai and Takuya Mizukami and Chisato Sato and Masaaki Kurata and Akihiro Umezawa and Seiji Shioda and Hiroshi Suzuki},
  journal={The Showa University Journal of Medical Sciences},
Abstract : Accumulating evidence from animal studies shows that the administration of mesenchymal stem cells (MSCs) from adult bone marrow ameliorates tissue damage after ischemic injury. In the present study we investigated the ef˜cacy of MSC implantation into a hindlimb ischemia model over a short-term period to elucidate the effects conferred within the early phase after treatment. MSCs from rats expressing green ˚uorescence protein (GFP) were injected into rat ischemic limbs. Laser Doppler… 

Figures from this paper

Human Umbilical Cord Stem Cells in Chitosan Attenuate Myocardial Injury in Rat Cardiac Infarction

Background: Myocardial infarction (MI) is the leading cause of cardiovascular deaths and disability in the industrialized world. Although stem cells have been injected into hearts to limit MI damage,

Effect of Peripheral Blood Mononuclear Cells on Induced Ischemia/Reperfusion in Skeletal Muscle of Adult Male Albino Rat: An Immunohistochemical Study

Evidence has suggested that reactive oxygen species (ROS) and inflammation play a crucial role in the pathogenesis of hind limb IRI (Kılıç et al., 2017).



Coronary vein infusion of multipotent stromal cells from bone marrow preserves cardiac function in swine ischemic cardiomyopathy via enhanced neovascularization

MSC infusion via the coronary vein prevented the progression of cardiac dysfunction in chronic MI, and this favorable effect appeared to derive not from cell differentiation, but from enhanced neovascularization by angiogenic factors secreted from the MSCs.

Mesenchymal stem cells: biology, pathophysiology, translational findings, and therapeutic implications for cardiac disease.

The current understanding of MSC biology, mechanism of action in cardiac repair, translational findings, and early clinical trial data of M SC therapy for cardiac disease are reviewed.

Local Delivery of Marrow-Derived Stromal Cells Augments Collateral Perfusion Through Paracrine Mechanisms

MSC injection improved limb function and appearance, reduced the incidence of auto-amputation, and attenuated muscle atrophy and fibrosis, suggesting MSCs can contribute to collateral remodeling through paracrine mechanisms.

Critical Roles of Muscle-Secreted Angiogenic Factors in Therapeutic Neovascularization

Implantation of IL-1&bgr;–deficient mononuclear cells improved tissue ischemia as efficiently as that of wild-type cells and stimulated muscle cells to produce angiogenic factors, thereby promoting neovascularization in ischemic tissues.

Effect of Hypoxia on Gene Expression of Bone Marrow‐Derived Mesenchymal Stem Cells and Mononuclear Cells

This work investigated the gene expression profiles between MSC and MNC of rat bone marrow under normoxia and hypoxia using a microarray containing 31,099 genes to provide information on the differential molecular mechanisms regulating the properties of MSCand MNC under ischemic conditions.