Membranes by the Numbers

@article{Phillips2018MembranesBT,
  title={Membranes by the Numbers},
  author={Rob Phillips},
  journal={arXiv: Biomolecules},
  year={2018},
  pages={73-105}
}
  • R. Phillips
  • Published 6 March 2017
  • Biology
  • arXiv: Biomolecules
Many of the most important processes in cells take place on and across membranes. With the rise of an impressive array of powerful quantitative methods for characterizing these membranes, it is an opportune time to reflect on the structure and function of membranes from the point of view of biological numeracy. To that end, in this chapter, I review the quantitative parameters that characterize the mechanical, electrical, and transport properties of membranes and carry out a number of… 
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References

SHOWING 1-10 OF 104 REFERENCES
Membrane lipids: where they are and how they behave
Throughout the biological world, a 30 Å hydrophobic film typically delimits the environments that serve as the margin between life and death for individual cells. Biochemical and biophysical findings
Organellar lipidomics--background and perspectives.
Lateral transport of a lipid probe and labeled proteins on a cell membrane.
TLDR
Diffusion coefficients (D) of a lipid probe and labeled proteins on L-6 myoblast membranes have been measured, showing some of the membrane proteins are immobile, but the lipid probe diffuses freely over macroscopic distances.
Configurations of fluid membranes and vesicles
Abstract Vesicles consisting of a bilayer membrane of amphiphilic lipid molecules are remarkably flexible surfaces that show an amazing variety of shapes of different symmetry and topology. Owing to
Theory of Passive Permeability through Lipid Bilayers
TLDR
A simple three-layer theory is presented that incorporates the area dependence in a physically realistic way and also includes the thickness as a secondary modulating parameter and the well-known strong correlation of permeability upon the partition coefficients of general solutes in hydrocarbon environments.
Thermodynamic analysis of the permeability of biological membranes to non-electrolytes.
Back to the future: mechanics and thermodynamics of lipid biomembranes.
TLDR
My aim is to provide some additional background, a kind of classroom ‘‘tutorial’’, regarding important mechanical and thermodynamic features of vesicle bilayers, lore established over the past two decades since Discussion 81, and highlight some new directions in the biophysics of membranes suggested by these insights.
A feeling for the numbers in biology
TLDR
The confluence of two different approaches to biological numbers is described and their synergism is illustrated in several particularly appealing case studies that reveal the impact that an emphasis on numbers can have on important biological questions.
Release of content through mechano-sensitive gates in pressurized liposomes
TLDR
It is shown that a single channel is able to relax the liposome, stressed to the point of bursting, in a matter of microseconds, and this provides a computational tool that opens the way to contribute to the rational design of functional nano-containers.
The variation of capacitance and conductance of bimolecular lipid membranes with area.
...
...