Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation.

Abstract

Neutrophil research relies largely on studies with highly purified cells. Yet the isolation procedures induce changes in surface expression of several proteins. We used a large panel of monoclonal antibodies (MoAbs) to characterize in detail the phenotypic changes during isolation and stimulation of human neutrophils. Centrifugation on density gradients… (More)

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