Membrane structures in normal and essential fatty acid-deficient stratum corneum: characterization by ruthenium tetroxide staining and x-ray diffraction.

@article{Hou1991MembraneSI,
  title={Membrane structures in normal and essential fatty acid-deficient stratum corneum: characterization by ruthenium tetroxide staining and x-ray diffraction.},
  author={S. Y. Hou and Ashim K Mitra and Stephen H White and Gopinathan K. Menon and Ruby Ghadially and Peter M Elias},
  journal={The Journal of investigative dermatology},
  year={1991},
  volume={96 2},
  pages={215-23}
}
Despite the importance of intercellular lamellar bilayers for stratum corneum (SC) barrier function, knowledge about the structure of these bilayers is limited due to their poor visualization and/or retention. Whereas substitution of ruthenium tetroxide (RuO4) for osmium tetroxide fixation provides clear images of these bilayers, the usefulness of RuO4 has been limited by its slow penetration and cytotoxicity. Utilizing a new fixation protocol for RuO4, we obtained clear images of lamellar… CONTINUE READING
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