Membrane structure of corneal epithelium: freeze-fracture observation.

  title={Membrane structure of corneal epithelium: freeze-fracture observation.},
  author={Minoru Tanaka and Yoshitaka Ohnishi and Taku Kuwabara},
  journal={Japanese journal of ophthalmology},
  volume={27 3},
Cytological details of the corneal epithelium were studied with special emphasis on the structure of the cell membrane. Normal corneas of various animals were fixed in a 4% solution of glutaraldehyde and divided into two portions. One-half was frozen with Freon 22 and freeze-fractured in frontal and sagittal directions using a Balzer instrument. The other… CONTINUE READING