Membrane stabilization during freezing: the role of two natural cryoprotectants, trehalose and proline.

@article{Rudolph1985MembraneSD,
  title={Membrane stabilization during freezing: the role of two natural cryoprotectants, trehalose and proline.},
  author={Alan S. Rudolph and John H. Crowe},
  journal={Cryobiology},
  year={1985},
  volume={22 4},
  pages={367-77}
}
The relative effectiveness of two natural cryoprotectants, proline and trehalose, in preserving membrane structure and function during freezing was studied. Isolated vesicles of sarcoplasmic reticulum (SR) from lobster muscle (Homarus americanus) were employed to study changes in structure and function during rapid freeze-thaw conditions. Both proline and trehalose were shown to effectively preserve the structure (assessed with freeze fracture) and function (assessed by the ability of the… CONTINUE READING
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