Membrane proteins and impure detergents: procedures to purify membrane proteins to a degree suitable for tryptophan fluorescence spectroscopy.

@article{Dijkstra1996MembranePA,
  title={Membrane proteins and impure detergents: procedures to purify membrane proteins to a degree suitable for tryptophan fluorescence spectroscopy.},
  author={Dolf Swaving Dijkstra and Jaap Broos and George Thomas Robillard},
  journal={Analytical biochemistry},
  year={1996},
  volume={240 1},
  pages={
          142-7
        }
}
Investigation of the membrane-embedded mannitol permease of Escherichia coli (EIImtl) steady-state tryptophan fluorescence was hampered by fluorescent impurities arising from detergents and other sources during the isolation. The signals from these impurities could not be distinguished from tryptophan fluorescence on the basis of lifetimes or emission spectra. Consequently, a tryptophan-minus mutant of EIImtl, EIImtl(Trp-), was constructed to address this problem. The findings were that the… CONTINUE READING
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