Membrane potential manipulation in synaptic plasma membrane vesicles for studying neurotransmitter uptake and release.


Synaptic plasma membrane (SPM) vesicles represent a membrane fraction very useful in studying non-vesicular neurotransmitter release. The procedure described here to isolate SPM vesicles from a crude synaptosomal fraction of sheep brain cortex is quick, simple (ultracentrifugation in a discontinuous density gradient of dextran T110), and combines a high… (More)


  • Presentations referencing similar topics