Membrane potential changes during pollen germination and tube growth

@article{Breygina2009MembranePC,
  title={Membrane potential changes during pollen germination and tube growth},
  author={Maria Breygina and Anatoly Smirnova and Niurguyana P Matveeva and I. P. Yermakov},
  journal={Cell and Tissue Biology},
  year={2009},
  volume={3},
  pages={573-582}
}
Using methods of quantitative fluorescent microscopy, we studied membrane potential changes during pollen germination and in growing pollen tubes. Two voltage-sensitive dyes were used, i.e., DiBAC4(3), to determine the mean membrane potential values in pollen grains and isolated protoplasts, and Di-4-ANEPPS, to map the membrane potential distribution on the surfaces of the pollen protoplast and pollen tube. We have shown that the activation of the tobacco pollen grain is accompanied by the… CONTINUE READING

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