Membrane permeability of bifunctional, amino site-specific, cross-linking reagents.

  title={Membrane permeability of bifunctional, amino site-specific, cross-linking reagents.},
  author={Tokichi Miyakawa and Larry J. Takemoto and C. Fred Fox},
  journal={Journal of supramolecular structure},
  volume={8 3},
The membrane permeability of a series of reversible cross-linking reagents which are diazide tartarate derivatives has been compared with that of dimethyl-3,3'-dithiobispropionimidate (DTBP). The diazide tartarate derivatives tested include tartryl-diazide (TDA), tartryl-di(glycylazide) (TDGA), tartryl-di(beta-alanylazide) (TDAA), tartryl-di-(gamma-aminobutyrylazide) (TDBA), tartryl-di(epsilon-aminocaproylazide) (TDCA). TDA, which has the shortest chain length of the diazide tartarate… 
6 Citations
Platelet protein organization: analysis by treatment with membrane-permeable cross-linking reagents.
Thrombin activation of platelets before treatment with diamide or DTSP results in increased cross-linking of myosin and increased incorporation of several proteins, particularly myos in and glycoproteins IIb and III, into high molecular weight material.
Selective Covalent Modification of Membrane Components
The search for means to locate and specifically characterize defined membrane constituents has intentionally been pursued and the approach offers obvious advantages in that (1) the extent and topology of label binding can be controlled chemically, and (2) label allocation is feasible at both the membrane and molecular level.
The band 3 protein of the human red cell membrane: a review.
  • T. Steck
  • Biology
    Journal of supramolecular structure
  • 1978
There is strong evidence that band 3 mediates 1:1 anion exchange across the membrane through a conformational cycle while remaining fixed and asymmetrical.
[51] Bifunctional reagents


A disulfide-bridge bifunctional imidoester as a reversible cross-linking reagent.
Spatial relationships of the proteins of vesicular stomatitis virus: induction of reversible oligomers by cleavable protein cross-linkers and oxidation
The data suggest that the glycoprotein spike of VS virus is composed of more than one G protein, consistent with the hypothesis that the matrix (M) protein may serve as a bridge between the G and N proteins in assembly of the VS virion.
Selective solubilization of proteins from red blood cell membranes by protein perturbants.
  • T. Steck, J. Yu
  • Biology, Chemistry
    Journal of supramolecular structure
  • 1973
The distinctive elution behavior which defines these two groups of polypeptides relates both to their chemical composition and their organizational disposition in the membrane.
The Behavior of Cleavable Crosslinking Reagents Based on the Disulfide Group
4,4′-Bisphenyldiazonium disulfide fluoroborate and dimethyl-3,3′-dithiobispropionimidate dihydrochloride were synthesized and tested as reversible cross-linking reagents to identify electrophoretically the components of crosslinked products.
Proteolytic dissection of band 3, the predominant transmembrane polypeptide of the human erythrocyte membrane.
Band 3 is the major, membrane-spanning, approximately90 000 dalton polypeptide of the human erythrocyte membrane and an interchain disulfide bond, which can be induced only at the cytoplasmic surface, cross-linked intact band 3, and certain of its water-soluble fragments.