Membrane permeability characteristics and osmotic tolerance limits of sea urchin (Evechinus chloroticus) eggs.

@article{Adams2003MembranePC,
  title={Membrane permeability characteristics and osmotic tolerance limits of sea urchin (Evechinus chloroticus) eggs.},
  author={Serean L Adams and Frederick W. Kleinhans and Philip V Mladenov and Paul A Hessian},
  journal={Cryobiology},
  year={2003},
  volume={47 1},
  pages={1-13}
}
Development of effective cryopreservation protocols relies on knowledge of the fundamental cryobiological characteristics for a particular cell type. These characteristics include osmotic behaviour, membrane permeability characteristics, and osmotic tolerance limits. Here, we report on measures of these characteristics for unfertilized and fertilised eggs of the sea urchin (Evechinus chloroticus). In NaCl solutions of varying osmolalities, sea urchin eggs behaved as ideal linear osmometers. The… CONTINUE READING

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