Membrane orientation and position of the C2 domain from cPLA2 by site-directed spin labeling.

@article{Frazier2002MembraneOA,
  title={Membrane orientation and position of the C2 domain from cPLA2 by site-directed spin labeling.},
  author={April A. Frazier and Mark A Wisner and Nathan J Malmberg and Kenneth G Victor and Gail Elizabeth Fanucci and Eric A. Nalefski and Joseph J. Falke and David S Cafiso},
  journal={Biochemistry},
  year={2002},
  volume={41 20},
  pages={6282-92}
}
The C2 domain is a ubiquitous Ca(2+)-binding motif that triggers the membrane docking of many key signaling proteins during intracellular Ca(2+) signals. Site-directed spin labeling was carried out on the C2 domain of cytosolic phospholipase A(2) in order to determine the depth of penetration and orientation of the domain at the membrane interface. Membrane depth parameters, Phi, were obtained by EPR spectroscopy for a series of selectively spin-labeled C2 domain cysteine mutants, and for spin… CONTINUE READING

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