Membrane mobility and microdomain association of the dopamine transporter studied with fluorescence correlation spectroscopy and fluorescence recovery after photobleaching.

Abstract

To investigate microdomain association of the dopamine transporter (DAT), we employed FCS (fluorescence correlation spectroscopy) and FRAP (fluorescence recovery after photobleaching). In non-neuronal cells (HEK293), FCS measurements revealed for the YFP-DAT (DAT tagged with yellow fluorescent protein) a diffusion coefficient (D) of approximately 3.6 x 10… (More)

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@article{Adkins2007MembraneMA, title={Membrane mobility and microdomain association of the dopamine transporter studied with fluorescence correlation spectroscopy and fluorescence recovery after photobleaching.}, author={Erika M Adkins and Devadoss John Samuvel and Jacob Ulrik Fog and Jacob Eriksen and Lankupalle Damodara Jayanthi and Christian Bjerggaard Vaegter and S. Ramamoorthy and Ulrik Gether}, journal={Biochemistry}, year={2007}, volume={46 37}, pages={10484-97} }