Membrane localization and dynamics of Nile Red: effect of cholesterol.

  title={Membrane localization and dynamics of Nile Red: effect of cholesterol.},
  author={Soumi Mukherjee and Hariharasundaram Raghuraman and Amitabha Chattopadhyay},
  journal={Biochimica et biophysica acta},
  volume={1768 1},
The organization and dynamics of the hydrophobic fluorescent probe Nile Red incorporated in DOPC vesicles containing varying amounts of cholesterol has been monitored utilizing fluorescence-based approaches which include the red edge excitation shift (REES) approach and the parallax method for depth determination. Our results show that the fluorescence emission maximum, intensity, polarization, and lifetime of Nile Red vary with the cholesterol content of the membrane. Interestingly, Nile Red… CONTINUE READING