Membrane insertion of small proteins.

  title={Membrane insertion of small proteins.},
  author={Andreas Kuhn and Natalie Stiegler and Anne-Kathrin Schubert},
  journal={Methods in molecular biology},
Proteins that are less than 10 kDa in size are easily purified under denaturing conditions and can often be refolded by removal of the denaturing agents. The purified small membrane proteins are competent for membrane insertion when the denaturing agent is diluted out and a membranous system like liposomes or proteoliposomes is added. This system allows the… CONTINUE READING