Membrane fusion plays an important role in gene transfection mediated by cationic liposomes

@article{Noguchi1998MembraneFP,
  title={Membrane fusion plays an important role in gene transfection mediated by cationic liposomes},
  author={Ari Noguchi and Tadahide Furuno and Chiyo Kawaura and Mamoru Nakanishi},
  journal={FEBS Letters},
  year={1998},
  volume={433}
}
By confocal laser scanning microscopy (CLSM) we have studied the membrane fusion between cationic liposomes and the endosome membranes involved in gene transfection mediated by cationic liposomes. Antisense oligonucleotides were transferred by cationic liposomes with a cationic cholesterol derivative, cholesteryl‐3β‐carboxyamidoethylenedimethylamine (I). Cationic liposomes were made by a mixture of the derivative I and DOPE. The intracellular distribution of fluorescein‐conjugated antisense… Expand
Confocal and probe microscopy to study gene transfection mediated by cationic liposomes with a cationic cholesterol derivative.
TLDR
A novel cationic cholesterol derivative with a hydroxyethyl amino head group (I) has been synthesized and used for liposome-mediated gene transfection and it was found that microtubules were involved in the intracellular dynamics of genetransfection. Expand
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TLDR
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TLDR
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PS exposure increases the susceptibility of cells to fusion with DOTAP liposomes.
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