Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome b5.

@article{Ravula2017MembraneED,
  title={Membrane environment drives cytochrome P450's spin transition and its interaction with cytochrome b5.},
  author={Thirupathi Ravula and C. Barnaba and M. Mahajan and G. Anantharamaiah and S. Im and L. Waskell and A. Ramamoorthy},
  journal={Chemical communications},
  year={2017},
  volume={53 95},
  pages={
          12798-12801
        }
}
Heme's spin-multiplicity is key in determining the enzymatic function of cytochrome P450 (cytP450). The origin of the low-spin state in ferric P450 is still under debate. Here, we report the first experimental demonstration of P450's membrane interaction altering its spin equilibrium which is accompanied by a stronger affinity for cytochrome b5. These results highlight the importance of lipid membrane for the function of P450. 
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Picturing the Membrane-assisted Choreography of Cytochrome P450 with Lipid Nanodiscs.
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  • Chemistry, Medicine
  • Chemphyschem : a European journal of chemical physics and physical chemistry
  • 2018
TLDR
The need to expand the use of nanodiscs, and the power of a combination of cutting-edge solution and solid-state NMR techniques, to study the dynamic structures of P450 as well as other membrane-proteins is highlighted. Expand
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