Membrane biosynthesis in the frog retina: opsin transport in the photoreceptor cell.

@article{Papermaster1975MembraneBI,
  title={Membrane biosynthesis in the frog retina: opsin transport in the photoreceptor cell.},
  author={David S. Papermaster and Carolyn A. Converse and J Siuss},
  journal={Biochemistry},
  year={1975},
  volume={14 7},
  pages={1343-52}
}
Rhodopsin biosynthesis and transport in the photoreceptor cell have been analyzed by subcellular fractionation of frog retinas after short periods of radioactive amino acid incorporation in vivo. Labelled membrane proteins were identified by autoradiography or sodium dodecyl sulfate-polyacrylamide gels. One of the most intensly labeled proteins in retina had a molecular weight comparable to opsin isolated from purified rod outer segments (ROS). Incorporation of label into this protein was rapid… CONTINUE READING