Membrane-binding properties of phospholipase C-β 1 and phospholipase C-β 2 : role of the C-terminus and effects of polyphosphoinositides , G-proteins and Ca 2 +

@inproceedings{Jenco1997MembranebindingPO,
  title={Membrane-binding properties of phospholipase C-β 1 and phospholipase C-β 2 : role of the C-terminus and effects of polyphosphoinositides , G-proteins and Ca 2 +},
  author={John Jenco and Kevin P Becker and Andrew J. Morris},
  year={1997}
}
We have studied the binding of two G-protein-regulated phospholipase C (PLC) enzymes, PLCs-β " and -β # , to membrane surfaces using sucrose-loaded bilayer phospholipid vesicles of varying compositions. Neither enzyme binds appreciably to pure phosphatidylcholine vesicles at lipid concentrations up to 10−$ M. PLC-β " and PLC-β # bind vesicles composed of phosphatidylcholine, phosphatidylserine and phosphatidylethanolamine (molar ratio 1:1 :1) with an approximate K d of 10−& M. Inclusion of 2… CONTINUE READING

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