Membrane binding and uptake of diflubenzuron in a cell line from Manduca sexta (L.)

  title={Membrane binding and uptake of diflubenzuron in a cell line from Manduca sexta (L.)},
  author={Gabriele E. Klitschka and Wolfgang Witt and Richard T. Mayer},
  journal={Archives of Insect Biochemistry and Physiology},
The binding and accumulation of the chitin synthesis inhibitor diflubenzuron (DFB) by a cell line derived from embryonic tissue of the tobacco hornworm, Manduca sexta (L.), was analyzed. A rapid and reversible binding to viable and nonviable cells suspended in the culture medium was observed at soluble concentrations of DFB for short exposure periods. Scatchard analysis gave no indication of a saturable uptake mechanism. The DFB-binding capacity of intact cells was found to be similar to that… Expand
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