Membrane association of Rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange

@article{Ullrich1994MembraneAO,
  title={Membrane association of Rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange},
  author={Oliver Ullrich and Hisanori Horiuchi and Cecilia Bucci and Marino Zerial},
  journal={Nature},
  year={1994},
  volume={368},
  pages={157-160}
}
THE Rab GTPases function as specific regulators of membrane transport1–4. The GTP/GDP cycle is believed to control shuttling of Rab proteins between the cytosol and organelle membranes. In vitro, Rab proteins are removed from membranes by a protein that inhibits GDP dissociation (rabGDI)5, which leads to formation of a cytosolic complex of Rab with the inhibitor protein6–8. Here we use a purified Rab5–rabGDI complex in a permeabilized cell system to investigate how the cytosolic complexed form… CONTINUE READING