Membrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a transmembrane protein.

@article{Katz1977MembraneAI,
  title={Membrane assembly in vitro: synthesis, glycosylation, and asymmetric insertion of a transmembrane protein.},
  author={Flora N Katz and James E Rothman and Vishwanath R. Lingappa and Gunter Blobel and Harvey F. Lodish},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1977},
  volume={74 8},
  pages={3278-82}
}
Membrane assembly was observed to proceed in cell-free extracts. Specifically, the membrane glycoprotein of vesicular stomatitis virus was synthesized in crude extracts of wheat germ in the presence of membrane vesicles derived from pancreatic endoplasmic reticulum. The resulting glycoprotein spans the lipid bilayer asymmetrically, is glycosylated, and is indistinguishable in these respects from the form of the glycoprotein found in the rough endoplasmic reticulum of virus-infected cells. Both… CONTINUE READING

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