Membrane-anchored Serine Protease Matriptase Is a Trigger of Pulmonary Fibrogenesis.

Abstract

RATIONALE Idiopathic pulmonary fibrosis (IPF) is a devastating disease that remains refractory to current therapies. OBJECTIVES To characterize the expression and activity of the membrane-anchored serine protease matriptase in IPF in humans and unravel its potential role in human and experimental pulmonary fibrogenesis. METHODS Matriptase expression was assessed in tissue specimens from patients with IPF versus control subjects using quantitative reverse transcriptase-polymerase chain reaction, immunohistochemistry, and Western blotting, while matriptase activity was monitored by fluorogenic substrate cleavage. Matriptase-induced fibroproliferative responses and the receptor involved were characterized in human primary pulmonary fibroblasts by Western blot, viability, and migration assays. In the murine model of bleomycin-induced pulmonary fibrosis, the consequences of matriptase depletion, either by using the pharmacological inhibitor camostat mesilate (CM), or by genetic down-regulation using matriptase hypomorphic mice, were characterized by quantification of secreted collagen and immunostainings. MEASUREMENTS AND MAIN RESULTS Matriptase expression and activity were up-regulated in IPF and bleomycin-induced pulmonary fibrosis. In cultured human pulmonary fibroblasts, matriptase expression was significantly induced by transforming growth factor-β. Furthermore, matriptase elicited signaling via protease-activated receptor-2 (PAR-2), and promoted fibroblast activation, proliferation, and migration. In the experimental bleomycin model, matriptase depletion, by the pharmacological inhibitor CM or by genetic down-regulation, diminished lung injury, collagen production, and transforming growth factor-β expression and signaling. CONCLUSIONS These results implicate increased matriptase expression and activity in the pathogenesis of pulmonary fibrosis in human IPF and in an experimental mouse model. Overall, targeting matriptase, or treatment by CM, which is already in clinical use for other diseases, may represent potential therapies for IPF.

DOI: 10.1164/rccm.201502-0299OC

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Cite this paper

@article{Bardou2016MembraneanchoredSP, title={Membrane-anchored Serine Protease Matriptase Is a Trigger of Pulmonary Fibrogenesis.}, author={Olivier Bardou and Awen Menou and Charl{\`e}ne François and Jan Willem Duitman and Jan H. von der Th{\"{u}sen and Raphael Borie and Katiuchia Uzzun Sales and Kathrin Mutze and Yves Castier and Edouard Sage and Ligong Liu and Thomas H Bugge and David P. Fairlie and Melanie K{\"{o}nigshoff and Bruno Crestani and Keren S Borensztajn}, journal={American journal of respiratory and critical care medicine}, year={2016}, volume={193 8}, pages={847-60} }