Membrane Interactions, Ligand-Dependent Dynamics, and Stability of Cytochrome P4503A4 in Lipid Nanodiscs.

  title={Membrane Interactions, Ligand-Dependent Dynamics, and Stability of Cytochrome P4503A4 in Lipid Nanodiscs.},
  author={N. A. Treuheit and Michelle Redhair and Hyewon Kwon and Wynton D McClary and M. Guttman and J. Sumida and W. Atkins},
  volume={55 7},
  • N. A. Treuheit, Michelle Redhair, +4 authors W. Atkins
  • Published 2016
  • Chemistry, Medicine
  • Biochemistry
  • Membrane-bound cytochrome P4503A4 (CYP3A4) is the major source of enzymatic drug metabolism. Although several structural models of CYP3A4 in various ligand complexes are available, none includes a lipid bilayer. Details of the effects of the membrane on protein dynamics and solvation, and access channels for ligands, remain uncertain. H/D exchange mass spectrometry (H/DXMS) with ligand free CYP3A4 containing a deletion of residues 3-12, compared to that of the full length wild type, in lipid… CONTINUE READING
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