Membrane Interactions, Ligand-Dependent Dynamics, and Stability of Cytochrome P4503A4 in Lipid Nanodiscs.

@article{Treuheit2016MembraneIL,
  title={Membrane Interactions, Ligand-Dependent Dynamics, and Stability of Cytochrome P4503A4 in Lipid Nanodiscs.},
  author={N. A. Treuheit and Michelle Redhair and Hyewon Kwon and Wynton D McClary and M. Guttman and J. Sumida and W. Atkins},
  journal={Biochemistry},
  year={2016},
  volume={55 7},
  pages={
          1058-69
        }
}
  • N. A. Treuheit, Michelle Redhair, +4 authors W. Atkins
  • Published 2016
  • Chemistry, Medicine
  • Biochemistry
  • Membrane-bound cytochrome P4503A4 (CYP3A4) is the major source of enzymatic drug metabolism. Although several structural models of CYP3A4 in various ligand complexes are available, none includes a lipid bilayer. Details of the effects of the membrane on protein dynamics and solvation, and access channels for ligands, remain uncertain. H/D exchange mass spectrometry (H/DXMS) with ligand free CYP3A4 containing a deletion of residues 3-12, compared to that of the full length wild type, in lipid… CONTINUE READING
    27 Citations

    Figures, Tables, and Topics from this paper

    Interactions of Cytochrome P450 3A4 with Phospholipid Bilayer Nanodiscs
    Picturing the Membrane-assisted Choreography of Cytochrome P450 with Lipid Nanodiscs.
    • C. Barnaba, A. Ramamoorthy
    • Chemistry, Medicine
    • Chemphyschem : a European journal of chemical physics and physical chemistry
    • 2018
    • 11
    • PDF
    Membrane-embedded substrate recognition by cytochrome P450 3A4
    • 10
    • PDF
    Computational Investigation of Ligand Binding to the Peripheral Site in CYP3A4: Conformational Dynamics and Inhibitor Discovery
    • 5
    Characterization of the structure and interactions of P450 BM3 using hybrid mass spectrometry approaches
    • 1
    • PDF

    References

    SHOWING 1-10 OF 55 REFERENCES
    Behavior of human cytochromes P450 on lipid membranes.
    • 70
    Ligand Binding to Cytochrome P450 3A4 in Phospholipid Bilayer Nanodiscs
    • 59
    • PDF
    Incorporation of charged residues in the CYP2J2 F-G loop disrupts CYP2J2-lipid bilayer interactions.
    • 28
    • PDF
    Structural differences between soluble and membrane bound cytochrome P450s.
    • 69
    Structural basis for ligand promiscuity in cytochrome P450 3A4
    • 546
    • PDF
    Homotropic cooperativity of monomeric cytochrome P450 3A4 in a nanoscale native bilayer environment.
    • 152
    Small-angle scattering determination of the shape and localization of human cytochrome P450 embedded in a phospholipid nanodisc environment.
    • 25
    • PDF