Membrane-Associated NAD-Dependent Isocitrate Dehydrogenase in Potato Mitochondria.

  title={Membrane-Associated NAD-Dependent Isocitrate Dehydrogenase in Potato Mitochondria.},
  author={George G. Laties},
  journal={Plant physiology},
  volume={72 4},
The oxidation isotherms for citrate and isocitrate by potato (Solanum tuberosum var. Russet Burbank) mitochondria in the presence of NAD differ markedly. Citrate oxidation shows positively cooperative kinetics with a sigmoid isotherm, whereas isocitrate oxidation shows Michaelis-Menten kinetics at concentrations up to 3 millimolar, and cooperative kinetics thereafter up to 30 millimolar. In the absence of exogenous NAD, the isocitrate isotherm is sigmoid throughout. The dual isotherm for… CONTINUE READING