Melting of a DNA helix terminus within the active site of a DNA polymerase.

@article{Hochstrasser1994MeltingOA,
  title={Melting of a DNA helix terminus within the active site of a DNA polymerase.},
  author={Remo Hochstrasser and Theodore E Carver and Lawrence C. Sowers and David P. Millar},
  journal={Biochemistry},
  year={1994},
  volume={33 39},
  pages={11971-9}
}
Accurate synthesis of DNA by polymerase is due in part to the selective removal of misincorporated nucleotides by a 3'-5' exonuclease activity (proofreading). Proofreading by an exonuclease domain containing a single-stranded DNA binding site may involve local melting of a duplex DNA substrate. Here we use time-resolved fluorescence spectroscopy to analyze the local melting of a DNA duplex terminus induced by the Klenow fragment of DNA polymerase I. Four oligodeoxynucleotide primer/templates… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 26 extracted citations

Similar Papers

Loading similar papers…