Melatonin antagonizes alpha-melanocyte-stimulating hormone enhancement of melanogenesis in mouse melanoma cells by blocking the hormone-induced accumulation of the c locus tyrosinase.

@article{Valverde1995MelatoninAA,
  title={Melatonin antagonizes alpha-melanocyte-stimulating hormone enhancement of melanogenesis in mouse melanoma cells by blocking the hormone-induced accumulation of the c locus tyrosinase.},
  author={P Valverde and E. Benedito and Francisco Solano and Sol Oaknin and Jos{\'e} Antonio Lozano and Jose Carlos Garc{\'i}a‐Borr{\'o}n},
  journal={European journal of biochemistry},
  year={1995},
  volume={232 1},
  pages={
          257-63
        }
}
Melatonin was found to have a small inhibitory effect on tyrosinase activity and a slight stimulatory action on dopachrome tautomerase activity in B16 mouse melanoma cells. These effects were time and dose dependent, with the maximal response being observed after 24-48 h treatment and at concentrations of melatonin higher than the physiologic levels of the circulating hormone. Although these effects on the melanogenic activities were modest, incubation of melanocytes with melatonin prior to the… Expand
Melatonin exerts oncostatic capacity and decreases melanogenesis in human MNT‐1 melanoma cells
TLDR
In vitro assessment indicates an oncostatic capacity of melatonin in time‐dependent manner in highly pigmented MNT‐1 melanoma cells and primary, electron paramagnetic resonance (EPR) spectroscopy demonstrated that melatonin reduced melanin content. Expand
Melatonin and its metabolites accumulate in the human epidermis in vivo and inhibit proliferation and tyrosinase activity in epidermal melanocytes in vitro
TLDR
Testing of their phenotypic effects in normal human melanocytes show that melatonin and its metabolites inhibit tyrosinase activity and cell growth, and inhibit DNA synthesis in a dose dependent manner with 10(-9) M being the lowest effective concentration. Expand
In Vitro Effects of Melatonin on Tumor Cells
TLDR
Melatonin, at physiological doses, inhibits cell proliferation and invasiveness of these cells, and lengthens the duration of the cell cycle, supporting the possible therapeutic value of melatonin in mammary cancer. Expand
Mitochondrial function is controlled by melatonin and its metabolites in vitro in human melanoma cells
TLDR
Results together with previously published reports provide a new biological potential and make an imperative to consider using melatonin or its metabolites for complementary future treatments of melanoma‐affected patients; however, these associations should be additionally investigated in clinical setting. Expand
Melanocortins and Melanoma
TLDR
This chapter reviews the literature of the past ten years by addressing the functional effects of MSH peptides on melanogenesis and intracellular signaling in melanoma cells as well as on cell proliferation and metastasis. Expand
The cutaneous serotoninergic/melatoninergic system: securing a place under the sun
  • A. Slominski, J. Wortsman, D. Tobin
  • Biology, Medicine
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2005
TLDR
The widespread expression of a cutaneous seorotoninergic/melatoninergic system indicates considerable selectivity of action to facilitate intra‐, auto‐, or paracrine mechanisms that define and influence skin function in a highly compartmentalized manner. Expand
Melatonin: A Cutaneous Perspective on its Production, Metabolism, and Functions.
TLDR
This review synthesizes the main features that render melatonin a promising candidate for the management of several dermatoses associated with substantial oxidative damage and why it promises to be useful in skin cancer prevention, skin photo- and radioprotection, and as an inducer of repair mechanisms that facilitate the recovery of human skin from environmental damage. Expand
Can melatonin and its metabolites boost the efficacy of targeted therapy in patients with advanced melanoma?
TLDR
It is hypothesize that melatonin and its metabolites could be promising boosters of the efficacy of BRAF/MEK inhibitors in patients with advanced melanoma. Expand
Melanin pigmentation in mammalian skin and its hormonal regulation.
TLDR
Melanogenesis is a highly structured system, active since early embryogenesis and capable of superselective functional regulation that may reach down to the cellular level represented by single melanocytes, and its significance extends beyond the mere assignment of a color trait. Expand
Alpha-MSH: Could this anti-inflammatory mediator be the culprit in Parkinson’s Disease?
TLDR
It is shown that key processes implicated in synuclein pathogenesis such as impairment of cellular autophagy and alpha-synuclein (α-syn) aggregation are induced by alpha-melanocyte stimulating hormone (alpha-MSH), a microglia-secreted anti-inflammatory mediator, which is consistent with PD pathology at the very early stage of disease. Expand
...
1
2
3
...

References

SHOWING 1-10 OF 46 REFERENCES
Melanin biosynthesis patterns following hormonal stimulation.
TLDR
It is shown that the MSH-induced stimulation of tyrosinase is accompanied by no significant change in the synthesis or catalytic activities of other melanogenic enzymes such as TRP1 or TRP2, which elicits a dramatic increase in melanin production accompanied by a significant decrease in the incorporation of carboxylated precursors into that melanin biopolymer. Expand
Alpha-melanocyte-stimulating hormone regulation of tyrosinase in Cloudman S-91 mouse melanoma cell cultures.
TLDR
Results suggest that in addition to stimulating tyrosinase synthesis, MSH may also promote an increase in the catalytic efficiency of the enzyme. Expand
Post-tyrosinase inhibition of melanogenesis by melatonin in hair follicles in vitro.
TLDR
In short-term (48 hr) culture hair follicles of the Siberian hamster retain both tyrosinase activity and the capacity to produce melanin, suggesting that the hair follicle melanocytes possess melatonin receptors, although their location remains to be determined. Expand
Human malignant melanoma cells express high-affinity receptors for melatonin: antiproliferative effects of melatonin and 6-chloromelatonin.
TLDR
Melatonin induced a marked concentration-dependent reduction in forskolin-stimulated cAMP accumulation in intact M-6 cells, indicating that it binds to a functional receptor in this cell line, which may be involved in mediation the antiproliferative effects of this hormone. Expand
Melatonin interactions with cultured murine B16 melanoma cells
TLDR
Cultured B16 murine melanoma cells possessed a specific, high affinity uptake system for melatonin which appeared to be altered by anti-oestrogen exposure. Expand
Tyrosinase isoenzymes in mammalian melanocytes. 2. Differential activation by alpha-melanocyte-stimulating hormone.
TLDR
In mouse melanoma melanocytes, alpha-melanocyte-stimulating hormone (MSH) stimulates differentiation, melanin synthesis and tyrosinase activity, which appears to be dependent on continued transcription and translation since it can be blocked by actinomycin and cycloheximide. Expand
α-MSH and Other Melanogenic Activators Mediate Opposite Effects of Tyrosinase and Dopachrome Tautomerase in B16/F10 Mouse Melanoma Cells
Abstract α-MSH was found to decrease the recently characterized dopachrome tautomerase activity in cultures of B16/F10 mouse melanoma cells. Other stimulating agents of melanogenesis, like dibutyrylExpand
In situ melanin assay for MSH using mouse B16 melanoma cells in culture.
TLDR
The new assay represents the most sensitive melanoma cell assay for MSH available to date, and shows that the peptides had identical intrinsic activities and that the relative potencies were similar to those obtained with a tyrosinase assay. Expand
Melatonin binding sites are functionally coupled to phosphoinositide hydrolysis in Syrian hamster RPMI 1846 melanoma cells.
TLDR
2-I-melatonin and N-acetylserotonin-induced phosphoinositide hydrolysis were not blocked by the serotonin type 2 antagonist ketanserin or alpha-adrenergic antagonist, phentolamine, and these data suggest that melatonin binding sites on RPMI 1846 cells are linked to phosphoinposide Hydrolysis as a second messenger. Expand
Synthesis and structure-function studies of melanocyte stimulating hormone analogues modified in the 2 and 4(7) positions: comparison of activities on frog skin melanophores and melanoma adenylate cyclase.
TLDR
There appears to be some differences in structural specificity at the melanotropin receptors of the two cell systems. Expand
...
1
2
3
4
5
...