Melatonin‐rich transgenic rice plants exhibit resistance to herbicide‐induced oxidative stress

  title={Melatonin‐rich transgenic rice plants exhibit resistance to herbicide‐induced oxidative stress},
  author={Sangkyu Park and Da-Eun Lee and Hyun-Jun Jang and Yeong Byeon and Young‐Soon Kim and Kyoungwhan Back},
  journal={Journal of Pineal Research},
To examine whether melatonin‐rich plants can defend against oxidative stress, we subjected melatonin‐rich transgenic (MRT) rice plants to the singlet‐oxygen‐generating herbicide butafenacil. Both MRT and transgenic control (TC; expressing the vector only) rice seeds germinated and grew equally well in continuous dark on half‐strength Murashige and Skoog (MS) medium containing 0.1 μm butafenacil. However, after transferring the seedlings to light, the TCs rapidly necrotized, whereas the MRT… 

Melatonin systemically ameliorates drought stress‐induced damage in Medicago sativa plants by modulating nitro‐oxidative homeostasis and proline metabolism

Novel results highlight the importance of melatonin as a promising priming agent for the enhancement of plant tolerance to drought conditions through the regulation of nitro‐oxidative and osmoprotective homeostasis.

Melatonin induction and its role in high light stress tolerance in Arabidopsis thaliana

  • H. LeeK. Back
  • Biology, Environmental Science
    Journal of pineal research
  • 2018
Melatonin was transiently induced in response to HL stress in Arabidopsis thaliana with a simultaneous increase in the expression of melatonin biosynthetic genes, including serotonin N‐acetyltransferase1 (SNAT1).

Elevated production of melatonin in transgenic rice seeds expressing rice tryptophan decarboxylase

To generate melatonin‐rich rice plants, three tryptophan decarboxylase isogenes in the rice genome were overexpressed through overexpression of TDC1, TDC2, and TDC3, resulting in increased melatonin content and enhanced levels of melatonin intermediates.

The ameliorative effects of exogenous melatonin on grape cuttings under water‐deficient stress: antioxidant metabolites, leaf anatomy, and chloroplast morphology

It is concluded that the application of melatonin to wine grapes is effective in reducing drought stress.

Overexpression of MzASMT improves melatonin production and enhances drought tolerance in transgenic Arabidopsis thaliana plants

The results elucidated the important role that membrane‐located melatonin synthase plays in drought tolerance and have significant implications in agriculture.

Exogenous melatonin application improves resistance to high manganese stress through regulating reactive oxygen species scavenging and ion homeostasis in tobacco

Melatonin (MT) can effectively improve the resistance of plants to various abiotic stresses. However, the effects of exogenous melatonin on the growth of tobacco plants under high manganese (Mn)

An increase in melatonin in transgenic rice causes pleiotropic phenotypes, including enhanced seedling growth, delayed flowering, and low grain yield

It is demonstrated that an alteration of endogenous melatonin levels leads to pleiotropic effects such as height, biomass, panicle number, flowering time, and grain yield, indicating that melatonin behaves as a signaling molecule in plant growth and reproduction.

Exogenous melatonin enhances salt stress tolerance in maize seedlings by improving antioxidant and photosynthetic capacity.

It is found that application of exogenous melatonin alleviated reactive oxygen species burst and protected the photosynthetic activity in maize seedlings under salt stress through the activation of antioxidant enzymes.

Melatonin Deficiency Confers Tolerance to Multiple Abiotic Stresses in Rice via Decreased Brassinosteroid Levels

The snat2 rice exhibited tolerance to multiple stress conditions including cadmium, salt, cold, and heat, as evidenced by decreased malondialdehyde (MDA) levels and increased chlorophyll levels, in contrast with SNAT2 overexpression lines, which were less tolerant to stress than wild type plants.



Melatonin promotes seminal root elongation and root growth in transgenic rice after germination

It is found that melatonin plays an important role in regulating both seminal root length and root growth after germination in monocotyledonous rice plants, and treatment with 0.5 and 1 μm melatonin promoted seminal root growth of the wild type under continuous light.

Lowering intercellular melatonin levels by transgenic analysis of indoleamine 2,3‐dioxygenase from rice in tomato plants

The results suggest the involvement of IDO in plant melatonin metabolism and a possible role in plant leaf development.

Presowing seed treatment with melatonin protects red cabbage seedlings against toxic copper ion concentrations

H, HM1 and HM10 improved seed germination both in water and in the presence of Cu2+.

Delayed senescence of apple leaves by exogenous melatonin treatment: toward regulating the ascorbate–glutathione cycle

H2O2 accumulation was inhibited by exogenous melatonin, which might have resulted from direct reactive oxygen species scavenging by melatonin and a great enhancement of ascorbate peroxidase (APX; EC, which acted on both mRNA and protein activity levels.

Enhanced production of melatonin by ectopic overexpression of human serotonin N‐acetyltransferase plays a role in cold resistance in transgenic rice seedlings

Experiments using both exogenous treatment of serotonin and senescent detached leaves, which contain a pool of serotonin, significantly enhancedmelatonin biosynthesis, indicating that endogenous serotonin levels play a bottleneck role in the pathway of melatonin biosynthetic.

[Genetic transformation of Nicotiana tabacum L. by Agrobacterium tumefaciens carrying genes in the melatonin biosynthesis pathway and the enhancement of antioxidative capability in transgenic plants].

Arylalkylamine N-acetyltransferase (AANAT) and Hydroxyindole O-methyltransferase(HIOMT) are the key regulation enzymes in the melatonin biosynthesis pathway in mammals. The AANAT and HIOMT genes were

Light‐regulated melatonin biosynthesis in rice during the senescence process in detached leaves

The results suggest that melatonin biosynthesis during senescence is dependent on light signals in rice leaves, contrary to the response found in animals.

Mechanism of growth amelioration of NaCl-stressed rice (Oryza sativa L.) by δ-aminolevulinic acid.

The mechanism of growth amelioration of NaCl-stressed rice (Oryza sativa L. cv. Nipponbare) by δ-aminolevulinic acid (ALA) was investigated. Rice growth recovered when plants were pretreated with 0.1

Melatonin promotes adventitious‐ and lateral root regeneration in etiolated hypocotyls of Lupinus albus L.

The physiological effect of exogenous melatonin as root promoter was demonstrated, its action being similar to that of indole‐3‐acetic acid.

Melatonin applied to cucumber (Cucumis sativus L.) seeds improves germination during chilling stress

Melatonin protected membrane structure against peroxidation during chilling, but excessive melatonin levels in cucumber seeds (∼4 μg/g fresh weight) provoked oxidative changes in proteins.