Melanosome uptake is associated with the proliferation and differentiation of keratinocytes

@article{Choi2013MelanosomeUI,
  title={Melanosome uptake is associated with the proliferation and differentiation of keratinocytes},
  author={Hye In Choi and Kyung-Cheol Sohn and Dongkyun Hong and Young Ho Lee and Chang Deok Kim and Tae‐Jin Yoon and Jin Woon Park and Sunggyun Jung and Jeung-Hoon Lee and Young Ho Lee},
  journal={Archives of Dermatological Research},
  year={2013},
  volume={306},
  pages={59-66}
}
Melanosomes are synthesized in melanocytes and transferred to neighboring keratinocytes. However, the associations of melanosome uptake with the proliferation and differentiation of keratinocytes are not fully understood. We examined the associations of melanosome uptake with keratinocyte differentiation and proliferation. SV40T-transformed human epidermal keratinocytes (SV-HEKs) were treated with isolated melanosomes. The effects of melanosome uptake on the proliferation and differentiation of… 

Melanin Uptake Reduces Cell Proliferation of Human Epidermal Keratinocytes

Together, the direct incubation of keratinocyte with melanin might serve as a useful model to study the potential mechanisms involved in melanin uptake and pigmentation process.

Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage

This study demonstrates that change in the proportion of melanocores in the intact/undegraded state by CTSV-related degradation in KCs affects photoprotection of the skin.

Human melanocytes mitigate keratinocyte-dependent contraction in an in vitro collagen contraction assay.

Low-concentration hydrogen peroxide can upregulate keratinocyte intracellular calcium and PAR-2 expression in a human keratinocyte–melanocyte co-culture system

Low H2O2 concentration promotes melanosome transfer with increased PAR-2 expression level and calcium concentration in keratinocytes, in which extracellular calcium and Par-2 were involved, and the interaction between melanocytes and keratinocyte is more beneficial to enhance calcium levels in Keratinocytes which mediate melanin transfer.

Stimulation of keratinocyte differentiation by extract of combined medicinal plant

ECMP applied topically to tape-stripped mouse skins accelerated reduction of transepidermal water loss (TEWL), indicating fast recovery of barrier function and Immunohistochemistry showed that ECMP increased the filaggrin level in tape-striped mouse skins, suggesting thatECMP may be applicable for keratinocyte differentiationrelated skin diseases.

Revisiting the role of melatonin in human melanocyte physiology: A skin context perspective

It is clinically and biologically important to definitively clarify whether melanocyte cell culture‐based observations translate into melatonin‐induced pigmentary changes in a physiological tissue context, that is, in human epidermis and hair follicles ex vivo, and are confirmed by clinical trial results.

Eumelanin-releasing spongy-like hydrogels for skin re-epithelialization purposes

Spongy-like hydrogels are proposed as sustained release matrices of S. officinalis eumelanin for predicting a beneficial role in skin wound healing through a direct effect over keratinocytes.

References

SHOWING 1-10 OF 23 REFERENCES

Keratinocytes in culture accumulate phagocytosed melanosomes in the perinuclear area

A new method is described that allows the spectrophotometric visualization of melanosome uptake by normal human keratinocytes in culture and suggests that the method is a useful model to quantitate keratinocyte phagocytosis of melanOSomes visually in vitro.

The protease-activated receptor 2 regulates pigmentation via keratinocyte-melanocyte interactions.

Evidence is provided that the protease-activated receptor 2 (PAR-2) expressed on keratinocytes, but not on melanocytes, is involved in melanosome transfer and therefore may regulate pigmentation.

Keratinocyte growth factor promotes melanosome transfer to keratinocytes.

Investigation of the influence of KGF on melanosome transfer in co-cultures of keratinocytes and melanocytes showed that KGF promotes phagocytosis through both mechanisms, suggesting a direct role of the receptor in regulating both the early steps of uptake and the intracellular traffic of the phagosomes.

Melanosomes are transferred from melanocytes to keratinocytes through the processes of packaging, release, uptake, and dispersion.

A melanosome transfer pathway wherein melanosomes are transferred from melanocytes to keratinocytes via the shedding vesicle system is suggested, which generates pigment globules containing multiple melanosites in a unique manner.

Melanosome transfer to and translocation in the keratinocyte

Ultraviolet irradiation (UVR) can modulate the process of melanosome transfer from the melanocytes to the keratinocytes and UVR can upregulate expression of PAR‐2 and lectin‐binding receptors and increase phagocytic activity of cultured keratinocyte.

Involvement of pigment globules containing multiple melanosomes in the transfer of melanosomes from melanocytes to keratinocytes

A novel mechanism of melanosome transfer is suggested, wherein melanosomes are packed in membrane globules that bud off from melanocyte dendrites, where they are released into the extracellular space and then phagocytosed by keratinocytes.

Inhibition of melanosome transfer results in skin lightening.

It is shown that modulation of protease-activated receptor 2 activation affects melanosome transfer into keratinocytes, resulting in changes in pigment production and deposition, and the use of RWJ-50353 to modulate prote enzyme-activated receptors 2 activation could lead to a new class of depigmenting agents.

Cellular mechanisms regulating human melanogenesis

The major differentiated function of melanocytes is the synthesis of melanin, a pigmented heteropolymer that is synthesized in specialized cellular organelles termed melanosomes, which are arranged in a supranuclear cap, protecting the DNA against incident ultraviolet light (UV) irradiation.

Expression and Functional Role of Sox9 in Human Epidermal Keratinocytes

Results suggest that Sox9 is an important regulator of epidermal keratinocytes with putative pro-proliferation and/or pro-survival functions, and may be related to several cutaneous diseases that are characterized by abnormal differentiation and hyperprol proliferation.

The expression and activation of protease-activated receptor-2 correlate with skin color.

It is shown that PAR-2 and trypsin are expressed in higher levels, and are differentially localized in highly pigmented, relative to lightly pigmented skins, and highly Pigmented skins exhibit an increase inPAR-2-specific protease cleavage ability.