Meiotic spindle imaging in human oocytes frozen with a slow freezing procedure involving high sucrose concentration.

@article{Bianchi2005MeioticSI,
  title={Meiotic spindle imaging in human oocytes frozen with a slow freezing procedure involving high sucrose concentration.},
  author={Veronica Bianchi and Giovanni Coticchio and Luca Fava and Carlo Flamigni and Andrea Borini},
  journal={Human reproduction},
  year={2005},
  volume={20 4},
  pages={1078-83}
}
BACKGROUND One of the major concerns derived from the cryopreservation of meiotically mature oocytes is possible damage to the cytoskeletal apparatus, and in particular the meiotic spindle. METHODS One hundred fresh oocytes showing the polar body I and high meiotic spindle birefringence (maximum retardance+/-1.5 mol/l SD = 2.58+/-0.1 nm), assessed through analysis, were included in this study. Oocytes were cryopreserved with a 1.5 mol/l 1,2-propanediol +0.3 mol/l sucrose solution. After… CONTINUE READING

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