Meiotic cell cycle arrest in mammalian oocytes

  title={Meiotic cell cycle arrest in mammalian oocytes},
  author={Anima Tripathi and K.V. Prem Kumar and Shail K Chaube},
  journal={Journal of Cellular Physiology},
Meiotic cell cycle in mammalian oocytes is a dynamic process that involves several stop/go channels. The cell cycle arrest in oocyte occurs at various stages such as diplotene, metaphase‐I (M‐I), metaphase‐II (M‐II), and so called metaphase‐like arrest (M‐III). Leutinizing hormone surge induces meiotic resumption from diplotene arrest in follicular microenvironment by overriding several factors responsible for the maintenance of meiotic arrest. The inhibitory factors are synthesized in oocyte… 

Cyclic nucleotides regulate oocyte meiotic maturation and quality in mammals

High level of cAMP as well as cGMP levels maintains diplotene arrest for a long time in follicular oocytes, thereby meiotic maturation process, which enables oocyte to achieve developmental competency.

Hormonal control of mammalian oocyte meiosis at diplotene stage

A detailed investigation of NPPC and NPR2 expression in follicle cells will elucidate the precise molecular mechanisms of gonadotropins, and control the arrest as well as resumption of meiosis.

Role of Mitogen Activated Protein Kinase and Maturation Promoting Factor During the Achievement of Meiotic Competency in Mammalian Oocytes

Further studies are required to find out the MAPK3/1 activity and its impact on MPF destabilization/stabilization during achievement of meiotic competency, an important period that decides oocyte quality and directly impacts ARTs outcome in several mammalian species including human.

Checkpoint kinase 1 is essential for meiotic cell cycle regulation in mouse oocytes

It is suggested that Chk1 is indispensable for prophase I arrest and functions in G2/M checkpoint regulation in meiotic oocytes and overexpression affects meiotic spindle assembly checkpoint regulation and thus chromosome segregation.

Maturation promoting factor destabilization mediates human chorionic gonadotropin induced meiotic resumption in rat oocytes

The data suggest that hCG surge increased cyclic nucleotides level in encircling granulosa cells but decreased their level in oocyte, and the changes in phosphorylation status of Cdk1 and reduced cyclin B1 level might have resulted in maturation promoting factor (MPF) destabilization.

Changes in signal molecules and maturation promoting factor levels associate with spontaneous resumption of meiosis in rat oocytes

The data suggest that the decrease of cAMP level and increase of cytosolic free Ca2+ as well as H2O2 levels associate with the reduced MPF level and meiotic resumption from diplotene arrest.

A New Understanding on the Regulation of Oocyte Meiotic Prophase Arrest and Resumption

A better understanding of these signaling networks on the regulation of oocyte meiotic progress will provide new opportunities for the manipulation of follicular functions for contraception or the treatment of infertility.

Role of granulosa cell mitogen-activated protein kinase 3/1 in gonadotropin-mediated meiotic resumption from diplotene arrest of mammalian oocytes

MAPK3/1 from GCs origin plays important role in gonadotropin-mediated meiotic resumption from diplotene arrest in follicular oocyte of mammals.

Journey of oocyte from metaphase‐I to metaphase‐II stage in mammals

Quality of oocyte directly impact fertilization rate, early embryonic development, and reproductive outcome in mammals, and oocyte journey from M‐I to M‐II stage is coordinated by several factors and pathways that enable oocyte to extrude PB‐I.



The metaphase II arrest in mouse oocytes is controlled through microtubule‐dependent destruction of cyclin B in the presence of CSF.

It is reported that in mouse oocytes a CSF‐like activity is involved in the M II arrest and that the high activity of MPF is maintained through a continuous equilibrium between cyclin B synthesis and degradation, which is also dependent upon the three‐dimensional organization of the spindle.

Meiotic Arrest in Human Oocytes Is Maintained by a Gs Signaling Pathway1

It is demonstrated that human oocytes maintain meiotic arrest prior to the LH surge using a signaling pathway similar to that of rodent oocytes.

Involvement of Mitogen-Activated Protein Kinase Cascade During Oocyte Maturation and Fertilization in Mammals1

Mitogen-activated protein kinase (MAPK) is a family of Ser/Thr protein kinases that are widely distributed in eukaryotic cells that plays pivotal roles in regulating the meiotic cell cycle progression of oocytes.

The signal pathway of gonadotrophins-induced mammalian oocyte meiotic resumption.

A detailed appreciation of different FSH and LH-activated signaling pathways in mammalian oocytes will be needed in understanding their actions in follicular development and oocyte maturation.

Stops and starts in mammalian oocytes: recent advances in understanding the regulation of meiotic arrest and oocyte maturation.

This review focuses on recent studies highlighting the importance of the oocyte in producing cAMP to maintain arrest, and discusses possible targets at the level of the Oocyte on which LH could act to stimulate meiotic resumption.

New Pathways from PKA to the Cdc2/cyclin B Complex in Oocytes: Wee1B as a Potential PKA Substrate

The biochemical steps linking a decrease in cAMP levels and MPF activation have been explored only recently and data supporting a simple scenario whereby Cdc25 and Wee1 kinase are substrates of the PKA in oocytes are reviewed.

The Anaphase-promoting Complex/Cyclosome Inhibitor Emi2 Is Essential for Meiotic but Not Mitotic Cell Cycles*

Fertilization triggers rapid, complete degradation of Emi2, but it is resynthesized in the first embryonic cell cycle to reach levels 5-fold lower than during CSF arrest, and depletion of the protein from cycling egg extracts does not prevent mitotic cell cycle progression.

Towards a new understanding on the regulation of mammalian oocyte meiosis resumption

A recent granulosa cell-specific knockout study has for the first time provided in vivo evidence for the important role of extracellular regulated kinase 1 and 2 (ERK1/2), two main forms of MAPK, and their downstream molecules ingranulosa cells in oocyte meiosis resumption.

Protein kinase A regulates resumption of meiosis by phosphorylation of Cdc25B in mammalian oocytes

The studies suggest that Cdc25B is a direct target of PKA in prophase-arrested oocytes and that CDC25B phosphorylation results in its inhibition and sequestration by the 14-3-3 protein.

Cyclic GMP from the surrounding somatic cells regulates cyclic AMP and meiosis in the mouse oocyte

It is found that cGMP passes through gap junctions into the oocyte, where it inhibits the hydrolysis of cAMP by the phosphodiesterase PDE3A, and causes a decrease in oocyte cAMP, leading to the resumption of meiosis.