Meiosis and fertility in interspecific hybrids of Nicotiana tabacum L. ×N. glauca Grah. and their derivatives

  title={Meiosis and fertility in interspecific hybrids of Nicotiana tabacum L. ×N. glauca Grah. and their derivatives},
  author={Anna Trojak-Goluch and Apoloniusz Berbe{\'c}},
  journal={Plant Breeding},
Nicotiana glauca is of potential interest to breeders as it carries resistance to black root rot of tobacco. Cytological investigations of sexual interspecific hybrids of N. tabacum T'T'TT (2n = 4x = 48) cv. 'Wislica' x N. glauca GG (2n - 2x = 24) were carried out. The analyses of chromosome association at diakinesis and metaphase I in the PMCs of amphihaploid F] T'TG (2n = 3x = 36) revealed low variable pairing with 0-5 bivalents. The sterile amphihaploids F 1 were converted into partial… 
Growth, development and chemical characteristics of tobacco lines carrying black root rot resistance derived from Nicotiana glauca (Grah.)
Results indicate that incorporation of the N. glauca-type resistance into tobacco genome did not produce negative effects on morphological and chemical traits.
Understanding meiosis and the implications for crop improvement.
This research has mainly focussed on the polyploid, bread wheat, and aims to introduce beneficial traits from wild related species to expand the use of non-adapted and wild germplasm in cereal breeding programs.
Polyploidy in Industrial Crops: Applications and Perspectives in Plant Breeding
Polyploidisation is used in plant breeding to overcome the non-viability and infertility of interspecific hybrids, obtain seedless polyploid cultivars and increase resistance/tolerance to biotic and abiotic factors.
Isolation and culture of protoplasts from anthers of apple (Malus pumila Mill.) rootstock ‘M9’
A reliable method for callus induction, isolation, and the culture of anther-derived protoplasts from the apple rootstock, ‘M9’ is described and information on the development of a protoplast-to-plant regeneration system is provided.
Assessing the bioconfinement potential of a Nicotiana hybrid platform for use in plant molecular farming applications
Hybrids exhibit extremely low fertility with no difference of green biomass yields relative to N. tabacum, and show potential to contribute to a bioconfinement- and biomanufacturing host system.
Pre-isolation, isolation and regeneration protoplasts from leaf mesophyll of in vivo Malus domestica ‘Anna’ cv.
The protocol might encourage the plant recovery using in apple somatic hybridization programs and diverse factors, for example, Osmotic pressure, incubation period, sieve pore size, centrifugation period and hormonal balance was estimated using the techniques for isolation.
Effectiveness of combining va-mediated resistance with a lettuce mosaic virus coat protein gene and a potato virus Y polymerase RNA gene in protection of tobacco hybrids from Potato virus Y
The most effective combination of va factor and transgene LMV CP was observed for VAM x MN 944 LMV and Wiślica x MN 900 LMV because a complete resistance was maintained in subsequent generations in the case of these hybrids.
An Interspecific Nicotiana Hybrid as a Useful and Cost-Effective Platform for Production of Animal Vaccines
It is demonstrated that injection with crude leaf extracts from these interspecific hybrid plants is a safe and effective approach for immunising mice, and these Nicotiana hybrids provide an advantageous production platform for partially purified, plant-made vaccines which may be particularly well suited for use in veterinary immunization programs.


Cytological investigations of the interspecific hybrids of Nicotiana tabacum L. x N. glauca Grah.
The number of univalents and the frequency of PMCs containing unpaired chromosomes indicated that amphidiploids N. tabacum cv.
Potential of Nicotiana glauca (Grah.) as a source of resistance to black root rot Thielaviopsis basicola (Berk. and Broome) Ferr. in tobacco improvement
A wide variation in response to black root rot was found for post-sesquidiploids a clear reflection of extensive chromosome segregation in that hybrid generation.
A study of morphology, cytology and sterility in interspecific hybrids and amphidiploids of Nicotiana knightiana X N. umbratica
SummaryInterspecific hybrids and amphidiploids of Nicotiana knightiana Goodspeed (n= 12)x N. umbratica Burbidge (n = 23) resembled either parent in some characters and were intermediate in other
Production of fertile transgenic Brassica napus by Agrobacterium-mediated transformation of protoplasts
Transformed protoplasts were regenerated to fertile and morphologically normal transgenic plants and genetic analysis of the progeny revealed that the kanamycin resistance introduced was inherited in a Mendelian fashion.