Megraft: a software package to graft ribosomal small subunit (16S/18S) fragments onto full-length sequences for accurate species richness and sequencing depth analysis in pyrosequencing-length metagenomes and similar environmental datasets.

Abstract

Metagenomic libraries represent subsamples of the total DNA found at a study site and offer unprecedented opportunities to study ecological and functional aspects of microbial communities. To examine the depth of a community sequencing effort, rarefaction analysis of the ribosomal small subunit (SSU/16S/18S) gene in the metagenome is usually performed. The… (More)
DOI: 10.1016/j.resmic.2012.07.001

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Cite this paper

@article{Bengtsson2012MegraftAS, title={Megraft: a software package to graft ribosomal small subunit (16S/18S) fragments onto full-length sequences for accurate species richness and sequencing depth analysis in pyrosequencing-length metagenomes and similar environmental datasets.}, author={Johan Bengtsson and Martin W. Hartmann and Martin Unterseher and Parag A. Vaishampayan and Kessy Abarenkov and Lisa M. Durso and Elisabeth M Bik and James R. Garey and K. Martin Eriksson and R. Henrik Nilsson}, journal={Research in microbiology}, year={2012}, volume={163 6-7}, pages={407-12} }