Megakaryocyte development and platelet production

  title={Megakaryocyte development and platelet production},
  author={Varda R. Deutsch and Aaron Tomer},
  journal={British Journal of Haematology},
Megakaryocytopoiesis involves the commitment of haematopoietic stem cells, and the proliferation, maturation and terminal differentiation of the megakaryocytic progenitors. Circulating levels of thrombopoietin (TPO), the primary growth‐factor for the megakaryocyte (MK) lineage, induce concentration–dependent proliferation and maturation of MK progenitors by binding to the c‐Mpl receptor and signalling induction. Decreased platelet turnover rates results in increased concentration of free TPO… 

Advances in megakaryocytopoiesis and thrombopoiesis: from bench to bedside

The regulation of megakaryocytopoiesis and platelet production in normal and disease states, and the innovative drugs and therapeutic modalities to stimulate or decrease thrombopoiedis are reviewed.

From hematopoietic stem cells to platelets

Summary.  Megakaryocytopoiesis is the process that leads to the production of platelets. This process involves the commitment of multipotent hematopoietic stem cells toward megakaryocyte (MK)

Shaping of terminal megakaryocyte differentiation and proplatelet development by sphingosine‐1‐phosphate receptor S1P4

  • S. GolfierS. Kondo M. Lipp
  • Biology
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2010
It is reported that sphin‐gosine 1‐phosphate receptor 4 (S1P4) is specifically up‐regulated during the development of human megakaryo‐cytes from progenitor cells and is expressed in mature murine megakARYocytes.

Bone marrow niche in immune thrombocytopenia: a focus on megakaryopoiesis

The interaction and role of BM niches in orchestrating megakaryopoiesis in ITP patients is examined and how these factors can be exploited to improve the quality of patient treatment and prognosis is discussed.

Development of Megakaryocytes

Key aspects of MK physiology and structure are discussed and the molecular pathways governing these fascinating cells under normal and some pathological conditions are explored.

Mapping the biogenesis of forward programmed megakaryocytes from induced pluripotent stem cells

This study has mapped parallels between the MKP states in vivo and in vitro and allowed the purification of MKPs, accelerating the progress of in vitro-derived transfusion products towards the clinic.

TPO-independent megakaryocytopoiesis.

OP9 Bone Marrow Stroma Cells Differentiate into Megakaryocytes and Platelets

It is revealed that mouse bone marrow stroma cell line OP9 cells differentiated into MKs and platelets in vitro, providing the first evidence for the differentiation of OP8 cells into MK's and platelet generation.

Understanding platelet generation from megakaryocytes: implications for in vitro-derived platelets.

The understanding of committed megakaryocytes and platelet release in vivo and in vitro, and how this knowledge can guide the development of in vitro-derived platelets for clinical application are focused on.



Molecular mechanisms of megakaryocyte differentiation.

This review focuses on the roles of selected transcription factors with key roles in MK differentiation, and on human and murine models of thrombocytopenia that result from impaired MK differentiation.

Thrombopoietin, the Mp1 ligand, is essential for full megakaryocyte development.

It is demonstrated that neutralizing the biological activity of Tpo eliminates MK formation in response to c-kit ligand, IL-6, and IL-11, alone and in combination, but that these reagents only partially reduceMK formation in the presence of combinations of cytokines including IL-3.

Megakaryocytopoiesis: characterization and regulation in normal and pathologic states.

Critical analysis of data indicates that megakaryocytopoiesis is a complex, multiple-stage cellular and biologic process that needs adequate interactions of several growth factors with target cell population and hematopoietic microenvironment.

The biogenesis of platelets from megakaryocyte proplatelets.

The process of platelet assembly is described in detail and several disorders that affect platelet production are discussed, including thrombocytopenia, which can lead to inadequate clot formation and increased risk of bleeding.

Megakaryocytes and beyond: the birth of platelets

Summary.  Megakaryocytes are highly specialized precursor cells that differentiate to produce blood platelets via intermediate cytoplasmic extensions known as proplatelets. Recent advances in the

Megakaryocyte maturation is associated with expression of the CXC chemokine connective tissue‐activating peptide CTAP III

High expression of CTAP III in mature human bone marrow MKs and megakaryoblast cell lines following differentiation induction with phorbol ester 12‐myristate 13‐acetate (PMA) is reported and molecular upregulation of CT AP III in MKs is associated with maturation and may be involved in proliferation arrest and cellular interactions with extracellular matrix and platelet production.

Megakaryocyte differentiation events.

The classification of functional categories of the cells of the megakaryocyte lineage is discussed, identifying those cells which respond to proliferative signals, those which mark the transition from the proliferating cell compartment to mature cells, and the mature, post-mitotic platelet-shedding cells.

Megakaryocyte differentiation events.

  • M. Long
  • Biology
    Seminars in hematology
  • 1998
The classification of functional categories of the cells of the megakaryocyte lineage is discussed, identifying those cells which respond to proliferative signals, those which mark the transition from the proliferating cell compartment to mature cells, and the mature, post-mitotic platelet-shedding cells.

In Vivo Platelet Production from Mature Megakaryocytes: Does Platelet Release Occur via Proplatelets?

  • G. Kōsaki
  • Biology
    International journal of hematology
  • 2005
A new “protoplatelet” concept is proposed to support the explosive-fragmentation theory and the role of the lungs in platelet production is reviewed and discussed.