Meet the neighbors: Mapping local protein interactomes by proximity‐dependent labeling with BioID

@inproceedings{Varnait2016MeetTN,
  title={Meet the neighbors: Mapping local protein interactomes by proximity‐dependent labeling with BioID},
  author={Renata Varnaitė and Stuart A MacNeill},
  booktitle={Proteomics},
  year={2016}
}
Proximity-dependent biotin identification (BioID) is a recently developed method that allows the identification of proteins in the close vicinity of a protein of interest in living cells. BioID relies on fusion of the protein of interest with a mutant form of the biotin ligase enzyme BirA (BirA*) that is capable of promiscuously biotinylating proximal proteins irrespective of whether these interact directly or indirectly with the fusion protein or are merely located in the same subcellular… CONTINUE READING
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